TNFSF9 Protein (AA 71-254) (His tag)
Quick Overview for TNFSF9 Protein (AA 71-254) (His tag) (ABIN667100)
Target
See all TNFSF9 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 71-254
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Purification tag / Conjugate
- This TNFSF9 protein is labelled with His tag.
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Sequence
- MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRE GPELSPDDPA GLLDLRQGMF AQLVAQNVLL IDGPLSWYSD PGLAGVSLTG GLSYKEDTKE LVVAKAGVYY VFFQLELRRV VAGEGSGSVS LALHLQPLRS AAGAAALALT VDLPPASSEA RNSAFGFQGR LLHLSAGQRL GVHLHTEARA RHAWQLTQGA TVLGLFRVTP EIPAGLPSPR SE
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Characteristics
- 4-1BB ligand , 71-254aa, Human, His-tagged, Recombinant, E.coli
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Purification
- > 95% by SDS-PAGE
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Endotoxin Level
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Measured by its binding ability in a functional ELISA with Human TNFRSF9 (CAT# ATGP3303).
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Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 71-254), (Trimer) (Active) protein (Fc Tag,DYKDDDDK Tag)
TNFSF9 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 71-254), (Trimer) (Active) protein (Fc Tag,Biotin,DYKDDDDK Tag)TNFSF9 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 71-254), (Trimer) (Active) protein (His-DYKDDDDK Tag)TNFSF9 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 68-251) (Active) protein (hFc Tag)TNFSF9 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 50-254) protein (His-DYKDDDDK-Strep II Tag)TNFSF9 Origin: Human Host: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 99-330) protein (His tag,mFc Tag)TNFSF9 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (Active) protein (His tag)TNFSF9 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 99-330) protein (Fc Tag)TNFSF9 Origin: Mouse Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 71-254) protein (His-IF2DI Tag)TNFSF9 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9) (AA 104-309) (Active) protein (Fc Tag)TNFSF9 Origin: Mouse Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 100 mM NaCl, 20 % glycerol
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Handling Advice
- Avoid repeated freezing and thawing cycles
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))
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Alternative Name
- 4-1BB Ligand/TNFSF9
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Background
- 4-1BB ligand is a type 2 transmembrane glycoprotein belonging to the TNF superfamily. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes. The interaction of 4-1BB with 4-1BBL strongly regulates immunity and has been proposed to preferentially control T cell responses based on studies in various murine models of cancer, infectious disease and autoimmune disease. Recombinant human 4-1BB ligand, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molecular Weight
- 23.8 kDa (222aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_003802
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Pathways
- Activated T Cell Proliferation, Cancer Immune Checkpoints
Target
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