TNFSF9 Protein (His tag)

Catalog No. ABIN7320663

Product Details

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Human Cells

Purification tag / Conjugate: This TNFSF9 protein is labelled with His tag.

Purpose: Recombinant Mouse TNFSF9 Protein (His Tag) (Active)

Sequence: Arg104-Glu309

Characteristics: Recombinant Mouse 4-1BB Ligand is produced by our Mammalian expression system and the target gene encoding Arg104-Glu309 is expressed with a 10His tag at the N-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Mouse 4-1BBL-His at 2μg/ml(100 μl/well) can bind Human 4-1BB-Fc(Cat: PKSH032026).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: TNFSF9 (Tumor Necrosis Factor (Ligand) Superfamily, Member 9 (TNFSF9))

Alternative Name: TNFSF9 (TNFSF9 Products)

Synonyms: TNFSF9 Protein, 4-1BB-L Protein, 4-1BBL Protein, AI848817 Protein, Cd137l Protein, Ly63l Protein, CD137L Protein, TNF superfamily member 9 Protein, tumor necrosis factor (ligand) superfamily, member 9 Protein, Tnfsf9 Protein, LOC476729 Protein, TNFSF9 Protein

Background:

Background: Tumor necrosis factor ligand superfamily member 9, also known as 4-1BBL, is a member of the the tumor necrosis factor family. Mouse 4-1BBL cDNA encodes a 309 amino acid residues (aa) protein with an 82 aa N-terminal cytoplasmic domain, a 21 aa transmembrane domain and a 206 aa C-terminal extracellular domain. The extracellular domain of 4-1BBL has a tertiary structure similar to that of other TNFSF members, but shares only low aa sequence homology (14-16 % ). 4-1BBL is predominantly expressed on activated antigen presenting cells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and B lymphoma cell lines. TNFSF9 has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells, and is thought to be involved in T cell-tumor cell interaction.

Synonym: Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL, Tnfsf9, Cd137l, Cd157l, Ly63l

Molecular Weight: 25.6 kDa

UniProt: P41274

Pathways: Activated T Cell Proliferation, Cancer Immune Checkpoints