ACAA1 Protein (AA 27-424) (His tag)
Quick Overview for ACAA1 Protein (AA 27-424) (His tag) (ABIN667183)
Target
See all ACAA1 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 27-424
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Purification tag / Conjugate
- This ACAA1 protein is labelled with His tag.
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Sequence
- MLSGAPQASA ADVVVVHGRR TAICRAGRGG FKDTTPDELL SAVMTAVLKD VNLRPEQLGD ICVGNVLQPG AGAIMARIAQ FLSDIPETVP LSTVNRQCSS GLQAVASIAG GIRNGSYDIG MACGVESMSL ADRGNPGNIT SRLMEKEKAR DCLIPMGITS ENVAERFGIS REKQDTFALA SQQKAARAQS KGCFQAEIVP VTTTVHDDKG TKRSITVTQD EGIRPSTTME GLAKLKPAFK KDGSTTAGNS SQVSDGAAAI LLARRSKAEE LGLPILGVLR SYAVVGVPPD IMGIGPAYAI PVALQKAGLT VSDVDIFEIN EAFASQAAYC VEKLRLPPEK VNPLGGAVAL GHPLGCTGAR QVITLLNELK RRGKRAYGVV SMCIGTGMGA AAVFEYPGN
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Characteristics
- ACAA1, 27-424aa, Human, His tag, E.coli
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Purification
- > 95% by SDS-PAGE
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Acetyl-CoA Acyltransferase 1 (ACAA1) (AA 1-424) protein (GST tag)
ACAA1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Acetyl-CoA Acyltransferase 1 (ACAA1) (AA 27-424) protein (His-IF2DI Tag)ACAA1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Acetyl-CoA Acyltransferase 1 (ACAA1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)ACAA1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Acetyl-CoA Acyltransferase 1 (ACAA1) (AA 27-331) protein (His tag)ACAA1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol 1 mM DTT, 0.1M NaCl
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Handling Advice
- Avoid repeated freezing and thawing cycles
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- ACAA1 (Acetyl-CoA Acyltransferase 1 (ACAA1))
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Alternative Name
- ACAA
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Background
- ACAA1 is a member of the thiolase family of enzymes and is involved in lipid metabolism. This protein is localized to the peroxisome and catalyzes the conversion of acyl-CoA and acetyl-CoA to 3-oxoacyl-CoA in the fatty acid oxidation pathway. ACAA1 shows high enzymatic activity in liver, kidney, intestine and white adipose tissue in rats. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Recombinant human ACAA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 43.8 kDa (419aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_001598
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Pathways
- Monocarboxylic Acid Catabolic Process
Target
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