DDAH1 Protein (AA 1-285) (His tag)

Catalog No. ABIN667244

This Recombinant DDAH1 protein is produced in Escherichia coli (E. coli).

Quick Overview for DDAH1 Protein (AA 1-285) (His tag) (ABIN667244)

Target: DDAH1 (Dimethylarginine Dimethylaminohydrolase 1 (DDAH1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-285

Purification tag / Conjugate: This DDAH1 protein is labelled with His tag.

Sequence: MAGLGHP AAFGRATHAV VRALPESLGQ HALRSAKGEE VDVARAERQH QLYVGVLGSK LGLQVVELPA DESLPDCVFV EDVAVVCEET ALITRPGAPS RRKEVDMMKE ALEKLQLNIV EMKDENATLD GGDVLFTGRE FFVGLSKRTN QRGAEILADT FKDYAVSTVP VADGLHLKSF CSMAGPNLIA IGSSESAQKA LKIMQQMSDH RYDKLTVPDD IAANCIYLNI PNKGHVLLHR TPEEYPESAK VYEKLKDHML IPVSMSELEK VDGLLTCCSV LINKKVDS

Characteristics: DDAH1, 1-285aa, Human, His tag, E.coli

Purification: > 95% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline ( pH 7.4) containing 10 % glycerol

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: DDAH1 (Dimethylarginine Dimethylaminohydrolase 1 (DDAH1))

Alternative Name: DDAH1

Background: Dimethylarginine dimethylaminohydrolase 1, also known DDAH1, belongs to the dimethylarginine dimethylaminohydrolase gene family. The DDAH1 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. Impairment of DDAH1 causes ADMA (asymmetric dimethylarginine) accumulation and a reduction in cGMP generation. Recombinant human DDAH1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 33.5 kDa (308aa) confirmed by MALDI-TOF

NCBI Accession: NP_036269