BPNT1 Protein (AA 1-308) (His tag)

Catalog No. ABIN667334

This Recombinant BPNT1 protein is produced in Escherichia coli (E. coli).

Quick Overview for BPNT1 Protein (AA 1-308) (His tag) (ABIN667334)

Target: BPNT1 (3'(2'), 5'-Bisphosphate Nucleotidase 1 (BPNT1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 %

Product Details

Protein Characteristics: AA 1-308

Purification tag / Conjugate: This BPNT1 protein is labelled with His tag.

Sequence: MASS NTVLMRLVAS AYSIAQKAGM IVRRVIAEGD LGIVEKTCAT DLQTKADRLA QMSICSSLAR KFPKLTIIGE EDLPSEEVDQ ELIEDSQWEE ILKQPCPSQY SAIKEEDLVV WVDPLDGTKE YTEGLLDNVT VLIGIAYEGK AIAGVINQPY YNYEAGPDAV LGRTIWGVLG LGAFGFQLKE VPAGKHIITT TRSHSNKLVT DCVAAMNPDA VLRVGGAGNK IIQLIEGKAS AYVFASPGCK KWDTCAPEVI LHAVGGKLTD IHGNVLQYHK DVKHMNSAGV LATLRNYDYY ASRVPESIKN ALVP

Characteristics: BPNT1, 1-308aa, Human, His tag, E.coli

Purification: > 90% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 5 mM DTT, 0.1M NaCl.

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: BPNT1 (3'(2'), 5'-Bisphosphate Nucleotidase 1 (BPNT1))

Alternative Name: BPNT1

Background: BPNT1, also known as BPntase, PAP phosphatase or PIP (PAP-inositol-1, 4-phosphatase), is a member of the magnesium-dependent, lithium-sensitive phosphomono-esterase superfamily. using magnesium as a cofactor, BPNT1 catalyzes the conversion of PAPS (adenosine 3'-phosphate 5' phosphosulfate) to APS (adenosine 5'-phosphosulfate) and the conversion of PAP (3' (2') -phosphoadenosine 5' phosphate) to AMP (adenosine 5'-phosphate). Expressed ubiquitously with highest levels in brain and kidney, BPNT1 is potently inhibited by lithium, a drug used for the treatment of manic depression and bipolar affective disorder, suggesting a possible role for BPNT1 in the etiology of mood disorders. Recombinant human BPNT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 37.5 kDa (344aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

NCBI Accession: NP_006076

Pathways: Inositol Metabolic Process