PHAP1 Protein (AA 1-249) (His tag)

Catalog No. ABIN667821

This Recombinant PHAP1 protein is expressed in Escherichia coli (E. coli).

Quick Overview for PHAP1 Protein (AA 1-249) (His tag) (ABIN667821)

Target: PHAP1 (ANP32A) (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-249

Purification tag / Conjugate: This PHAP1 protein is labelled with His tag.

Sequence: MGSSHHHHHH SSGLVPRGSH MEMGRRIHLE LRNRTPSDVK ELVLDNSRSN EGKLEGLTDE FEELEFLSTI NVGLTSIANL PKLNKLKKLE LSDNRVSGGL EVLAEKCPNL THLNLSGNKI KDLSTIEPLK KLENLKSLDL FNCEVTNLND YRENVFKLLP QLTYLDGYDR DDKEAPDSDA EGYVEGLDDE EEDEDEEEYD EDAQVVEDEE DEDEEEEGEE EDVSGEEEED EEGYNDGEVD DEEDEEELGE EERGQKRKRE PEDEGEDDD

Characteristics: ANP32A, 1-249aa, Human, His tag, E.coli

Purification: > 95% by SDS-PAGE

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 1 mM DTT, 100 mM NaCl, 0.1 mM PMSF

Handling Advice: Avoid repeated freezing and thawing cycles

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Target Details

Target: PHAP1 (ANP32A) (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A))

Alternative Name: ANP32A

Background: ANP32A (acidic leucine-rich nuclear phosphoprotein 32 family member A) has been shown to interact with MAP1B, TAF1A and Protein SET. This protein Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis. Also, it plays a role in E4F1-mediated transcriptional repression. Recombinant human ANP32A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 30.7 kDa (269aa) confirmed by MALDI-TOF

NCBI Accession: NP_006296