Nanog Protein (AA 1-154) (His tag)
Quick Overview for Nanog Protein (AA 1-154) (His tag) (ABIN667930)
Target
See all Nanog (NANOG) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-154
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Purification tag / Conjugate
- This Nanog protein is labelled with His tag.
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Sequence
- MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQ
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Characteristics
- NANOG, 1-154aa, Human, His tag, E.coli
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Purification
- > 90% by SDS-PAGE
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Nanog Homeobox (NANOG) (AA 2-305) protein (His-IF2DI Tag)
NANOG Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Nanog Homeobox (NANOG) (AA 1-305) proteinNANOG Origin: Human Host: Escherichia coli (E. coli) Recombinant > 80 % SDS
Nanog Homeobox (NANOG) (AA 1-305) proteinNANOG Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 1 mM DTT
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Handling Advice
- Avoid repeated freezing and thawing cycles
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- Nanog (NANOG) (Nanog Homeobox (NANOG))
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Alternative Name
- Nanog
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Background
- NANOG, also known as nanog homeobox, is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is down-regulated, cell differentiation can proceed. Recombinant human NANOG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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Molecular Weight
- 19.6 kDa (174aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
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NCBI Accession
- NP_079141
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Pathways
- Stem Cell Maintenance
Target
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