Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 530-826) Peptide

Catalog No. ABIN934906

Quick Overview for Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (AA 530-826) Peptide (ABIN934906)

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Product Details

Peptide Type: Recombinant

Protein Region: AA 530-826

Sequence: MEFKLELVEK LFAEDTEAKN PFSTQDTDLD LEMLAPYIPM DDDFQLRSFD QLSPLESSSA SPESASPQST VTVFQQTQIQ EPTANATTTT ATTDELKTVT KDRMEDIKIL IASPSPTHIH KETTSATSSP YRDTQSRTAS PNRAGKGVIE QTEKSHPRSP NVLSVALSQR TTVPEEELNP KILALQNAQR KRKMEHDGSL FQAVGIGTLL QQPDDHAATT SLSWKRVKGC KSSEQNGMEQ KTIILIPSDL ACRLLGQSMD ESGLPQLTSY DCEVNAPIQG SRNLLQGEEL LRALDQVN

Characteristics: Purified recombinant Human Hif1 a? protein
Expression System: E.coli
The recombinant protein appeared as a 40 kDa band on SDS-PAGE.

Purity: > 95 % pure

Application Details

Application Notes: Hif1-alpha protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris-HCl, pH7.5, containing 0 mM DTT.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))

Background: Hypoxia-inducible factor-1 (HIF-1), identified as one of the transcription factors, has been found to play an essential role in oxygen homeostasis. HIF-1 is a heterodimer composed of HIF-1 beta subunit and one of three subunits (Hif-1alpha, Hif-2alpha or Hif-3alpha). The activation of Hif-1 alpha is closely associated with a variety of tumors and oncogenic pathways. Hif-1 alpha consists of DNA binding domain (DBD domain), Dimerization domain and C-terminal regulatiory domains, including two transactivation domains (TAD), an oxygen-dependent degradation(ODD) domain, and inhibitory domains. Hif-1 alpha (530-826 residues) contains two TAD and inhibitory domain. Recombinant Hif-1 alpha (530-826 residues) was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: Hifa-1 protein, Hif1a, HIF 1A protein, Hifa 1 protein, ARNT interacting protein protein, MOP1 protein, HIF 1alpha protein, bHLHe78 protein, Hif-1 alpha (530-826 aa) protein, HIF-1 alpha protein, Member of PAS protein 1 protein, Hifa-1, Hypoxia-inducible factor-1a Basic helix loop helix PAS protein MOP1 protein, MOP 1 protein, Member of the PAS Superfamily 1 protein, PASD8 protein, Hypoxia inducible factor 1 alpha isoform I.3 protein, Basic-helix-loop-helix-PAS protein MOP1 protein, Hypoxia-inducible factor 1 alpha protein, HIF1A protein, HIF1 alpha protein, PASD 8 protein, , Member of PAS protein 1 protein, Member of PAS superfamily 1 protein, Hifa 1, ARNT-interacting protein protein, Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor protein, Hypoxia inducible factor 1 alpha subunit protein, HIF1 A protein, Hypoxia inducible factor 1 alpha protein

Molecular Weight: 32.8 kDa (298 AA)