AKR1B1 Protein (AA 1-316)

Catalog No. ABIN935005

Recombinant AKR1B1 protein expressed in Escherichia coli (E. coli).

Quick Overview for AKR1B1 Protein (AA 1-316) (ABIN935005)

Target: AKR1B1 (Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % pure

Product Details

Protein Characteristics: AA 1-316

Sequence: MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF

Characteristics: Purified recombinant Human Aldose reductase protein
Expression System: E.coli
Bioactivity: Specific activity: approximately 0.5-0.6 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 uM NADPH/min at 25 °C.
Molecular weight on SDS-PAGE will appear higher.

Endotoxin Level: < 1.0 EU per µg of protein (determined by LAL method)

Application Details

Application Notes: Aldose reductase protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Assay Procedure:

1. Prepare a 1 mL reaction mix into a suitable container : The final concentrations are 0.1 M sodium phosphate (pH 7.0), 11 mM DL-glyceraldehyde, 0.1 mM NADPH.
   2. Add 50 µL of recombinant AKR1B1 solution with various concentrations (2.5 µg, 5 µg, 15 µg) in 750 µL reaction buffer.
   3. Mix by inversion and Incubate at 25 °C for
   2.5 minutes.
   4. Add 200 µL of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion.
   5. Record the increase at A340 nm for 3 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT, and 10 % glycerol.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: AKR1B1 (Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1))

Alternative Name: Aldose reductase

Background: Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Recombinant Aldose reductase (AKR1B) protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: ALR2 protein, member B1 AKR1B 1 protein, ALDR1 protein, aldehyde reductase 1 protein, aldo-keto reductase family 1 protein, AKR1B1 protein, Aldo-keto reductase family1 member B1 protein, AR protein, aldr-1 protein, Lii5-2 CTCL tumor protein protein, ADR protein, Aldose reductase protein, member B1 aldr 1 protein, Aldo-keto reductase family1 protein, low Km aldose reductase., Aldehyde reductase protein

Molecular Weight: 35.8 kDa (316 AA)

Pathways: Metabolism of Steroid Hormones and Vitamin D, C21-Steroid Hormone Metabolic Process, Monocarboxylic Acid Catabolic Process