FAS Protein (Soluble)

Catalog No. ABIN967460

This Recombinant FAS protein is produced in Insect Cells. There are 3 publications available.

Quick Overview for FAS Protein (Soluble) (ABIN967460)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Protein Type: Recombinant

Origin: Human

Source: Insect Cells

Application: Western Blotting (WB), Standard (STD)

Product Details

Protein Characteristics: Soluble

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Licensed for Research Purposes Only. Commercial use requires license from Boyce Thompson Institute for Plant Research.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: Recombinant soluble human Fas was expressed in the Baculovirus Expression Vector System and purified from insect cell lysate using an anti-Fas antibody. This form of Fas lacks the transmembrane domain of the Fas receptor, and has been referred to as FasΔTM. It has a 63-base pair deletion starting at nucleotide sequence (700) GA TCC AGA and ending at nucleotide sequence GTT TGG G (762). The purity and the integrity of the protein were verified by SDS-PAGE, and ELISA. FasΔTM has a reduced molecular weight of ~40 kDa.

Application Details

Application Notes: Purified FasDeltaTM can be used as a positive control for western blot analysis of Fas. 1 µg of protein can easily be visualized on a Commasie-stained gel as a band of ~40 kDa. For western blot analysis, 1 µg or less is sufficient depending on the detection method (less is needed for enhanced chemiluminescence than for standard alkaline phosphatase detection). Purified FasDeltaTM can also be used as a standard in Fas ELISA assays. The recombinant FasDeltaTM protein should not be used in functional assays, as it does not appear to assume a biologically active conformation.

Comment:

BD Pharmingen™ Recombinant Soluble Human Fas (FasδTM)

Restrictions: For Research Use only

Handling

Format: Liquid

Handling Advice: Fas (APO-1, CD95) is an ~42 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary and lung. FasL is a 40 kDa TNF family member membrane protein that induces apoptosis by binding to Fas, its cell-surface receptor. FasL is expressed on activated T and NK cells. Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development.

Storage: -20 °C,-80 °C

Storage Comment: Avoid multiple freeze-thaws of product. Store the protein at -20°C, or for longer storage life, at -80°C.

References

Tanaka, Suda, Takahashi, Nagata: "Expression of the functional soluble form of human fas ligand in activated lymphocytes." in: The EMBO journal, Vol. 14, Issue 6, pp. 1129-35, (1995) (PubMed).

Cheng, Zhou, Liu, Shapiro, Brauer, Kiefer, Barr, Mountz: "Protection from Fas-mediated apoptosis by a soluble form of the Fas molecule." in: Science (New York, N.Y.), Vol. 263, Issue 5154, pp. 1759-62, (1994) (PubMed).

Takahashi, Tanaka, Brannan, Jenkins, Copeland, Suda, Nagata: "Generalized lymphoproliferative disease in mice, caused by a point mutation in the Fas ligand." in: Cell, Vol. 76, Issue 6, pp. 969-76, (1994) (PubMed).

Target Details

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: Fas

Background: Fas (APO-1, CD95) is an ~42 kDa cell surface protein that mediates apoptosis when cross-linked with agonistic anti-Fas antibodies or Fas ligand (FasL). Fas belongs to the TNF (tumor necrosis factor)/NGF (nerve growth factor) receptor family, and is expressed in various tissue and cells including the thymus, liver, ovary and lung. FasL is a 40 kDa TNF family member membrane protein that induces apoptosis by binding to Fas, its cell-surface receptor. FasL is expressed on activated T and NK cells. Both Fas and FasL are thought to play an important role in the apoptotic processes that take place during T cell development.

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity