anti-CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL) Antibodies

CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. Additionally we are shipping CTCFL Kits (8) and CTCFL Proteins (5) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
CTCFL 140690 Q8NI51
CTCFL 664799 A2APF3
CTCFL 100360757  
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Top anti-CTCFL Antibodies at antibodies-online.com

Showing 10 out of 89 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p) Immunocytochemistry/Immunofluorescence: BORIS Antibody [NBP1-89947] - Staining of human cell line U-251MG shows positivity in nucleus & cytoplasm. Immunohistochemistry-Paraffin: BORIS Antibody - Staining of human testis shows moderate nuclear positivity in subset of cells in seminiferous ducts. 0.1 mL Log in to see 10 to 13 Days
$491.63
Details
Human Rabbit Un-conjugated WB WB Suggested Anti-CTCFL Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:312500 Positive Control: Jurkat cell lysate CTCFL is supported by BioGPS gene expression data to be expressed in Jurkat WB Suggested Anti-CTCFL <br /> Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500 <br /> Positive Control: Jurkat cell lysate <br /> CTCFL is supported by BioGPS gene expression data to be expressed in Jurkat 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated ELISA, WB Western blot using Affinity Purified anti-BORIS antibody shows detection of a predominant band corresponding to BORIS in human tissue lysates (arrowhead).   Lane 1 contains lysate from human prostate tissue.  Lane 2 contains lysate from human spleen tissue.  A predominant band at ~75 kDa is observed.  Molecular weight estimation was made by comparison to prestained MW markers as indicated. 100 μg Log in to see 7 to 9 Days
$389.00
Details
Human Rabbit Un-conjugated WB 100 μg Log in to see 2 to 3 Days
$255.00
Details
Human Rabbit Un-conjugated WB 50 μg Log in to see 7 to 9 Days
$551.83
Details
Chimpanzee Rabbit Un-conjugated EIA, WB   0.1 mg Log in to see 8 to 11 Days
$619.30
Details
Human Rabbit Un-conjugated WB 50 μg Log in to see 7 to 9 Days
$551.83
Details
Human Mouse Un-conjugated ChIP, ELISA, ICC, IF, IHC, IP, WB Immunoprecipitation: BORIS Antibody (20B11) [NBP2-52405] - The 83-kDa protein can be immunoprecipitated with the mouse anti- BORIS C terminal specific antibody. Proteins were immunoprecipitated from the MCF7 cell extracts with the mouse anti- BORIS C terminal antibody 20B11 in their original supernatant form using the following volumes: (1) 400 ul of 20B11; (2) 800 ul of 20B11; (3) Input; (4) MCF7 cell lysates as BORIS positive control. The extracts from MCF7 cells were incubated overnight at 40C followed by 2 hour incubation with protein G sepharose beads. Proteins were resolved by SDS-PAGE, and Western blot analysis was performed with the concentrated form of the mouse anti- BORIS N terminal (4A7) specific antibody (1:500). Immunohistochemistry: BORIS Antibody (20B11) [NBP2-52405] - Analysis using the supernatants from the clone 20B11 at following dilutions: (A) 20B11 undiluted supernatant and (B) 20B11 at 1:25. (B) Cells have been counterstained with the nuclear marker hematoxylin (blue). Images were taken at x60 magnification using the Olympus BX41 microscope. Expression of BORIS protein is indicated by the presence of brown staining, with a pattern characteristic for BORIS ( both, cytoplasmic and nuclear). 0.1 mL Log in to see 7 to 9 Days
$373.75
Details
Human Rabbit Un-conjugated ELISA, WB Western Blot: BORIS Antibody [ABIN151496] - Analysis of a predominant band corresponding to BORIS in human tissue lysates (arrowhead). Lane 1 contains lysate from human prostate tissue. Lane 2 contains lysate from human spleen tissue. A predominant band at 75 kDa is observed. 0.1 mg Log in to see 7 to 9 Days
$585.06
Details
Human Rabbit Un-conjugated IC, IF, WB Immunofluorescent analysis of BORIS staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody Western blot analysis of BORIS expression in Jurkat (A), Hela (B), mouse heart (C), rat testis (D) whole cell lysates. 200 μL Log in to see 13 to 14 Days
$487.50
Details

Top referenced anti-CTCFL Antibodies

  1. Human Polyclonal CTCFL Primary Antibody for ICC, IF - ABIN4285064 : Hines, Bazarov, Mukhopadhyay, Yaswen: BORIS (CTCFL) is not expressed in most human breast cell lines and high grade breast carcinomas. in PLoS ONE 2010 (PubMed)
    Show all 3 Pubmed References

  2. Human Polyclonal CTCFL Primary Antibody for ELISA, WB - ABIN151496 : Klenova, Morse, Ohlsson, Lobanenkov: The novel BORIS + CTCF gene family is uniquely involved in the epigenetics of normal biology and cancer. in Seminars in cancer biology 2002 (PubMed)
    Show all 2 Pubmed References

  3. Human Polyclonal CTCFL Primary Antibody for ELISA, WB - ABIN129668 : Dougherty, Ichim, Liu, Reznik, Min, Ghochikyan, Agadjanyan, Reznik: Selective apoptosis of breast cancer cells by siRNA targeting of BORIS. in Biochemical and biophysical research communications 2008 (PubMed)
    Show all 2 Pubmed References

  4. Human Polyclonal CTCFL Primary Antibody for WB - ABIN2775057 : Sun, Huang, Nguyen, Bisht, Bar-Sela, Ho, Bradbury, Yu, Cui, Lee, Trepel, Feinberg, Gius: DNA methyltransferase 1 and 3B activate BAG-1 expression via recruitment of CTCFL/BORIS and modulation of promoter histone methylation. in Cancer research 2008 (PubMed)

More Antibodies against CTCFL Interaction Partners

Human CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL) interaction partners

  1. These findings highlight the role of intragenic DNA methylation (show HELLS Antibodies) and DNA binding protein (show UBE2V1 Antibodies) BORIS in cancer-specific splicing and its role in tumorigenesis.

  2. The CCCTC-binding factor (show CTCF Antibodies) plays critical roles in genome topology and function, increased risk of carcinogenicity, and potential of lung cancer-specific mediations. Chromosome reposition in lung cancer can be regulated by CCCTC binding factor (show CTCF Antibodies)

  3. These data provide information on the organization of the BORIS promoter region and gene regulation in normal and cancer cells. In addition, the study proposes that specific alleles of the BORIS-MS2 (show FAR2 Antibodies) region could be used to identify the risk for lung cancer.

  4. the results indicate that the CT antigen BORIS sf6 is specifically expressed in cervical cancer stem-like cells and cancer-initiating cells

  5. BORIS is associated with the cancer stem cell-like traits of human liver cancer cells through the epigenetic regulation of OCT4 (show POU5F1 Antibodies).

  6. In this study, the methylation status of BORIS was tested by methylation specific polymerase chain reaction in human hepatocarcinoma (HCC (show FAM126A Antibodies)) cell lines and 43 pairs of tissue specimens. Frequently demethylation of BORIS in HCC (show FAM126A Antibodies) was significantly higher than that in the paired adjacent non-tumor tissues (P=0.019), and it was correlated with tumor size (P=0.025) and clinical TNM (show ODZ1 Antibodies) stage (P=0.035).

  7. Effectively controlling BORIS/CTCFL levels can inhibit disease establishment and hence can be considered as a potent target for cancer therapy

  8. BORIS is associated with cancer stem cell-enriched populations of several epithelial tumor cells and the different phenotypes depend on the origin of tumor cells.

  9. Data found that BORIS and CTCF (show CTCF Antibodies) expression in low-grade squamous intraepithelial lesions and invasive cervical carcinoma is higher than in normal samples. The possible utility of BORIS and CTCF (show CTCF Antibodies) as biomarkers in cervical neoplasm requires further analysis.

  10. In the first detailed analysis in cancer, a marked loss of CHD8 (show CHD8 Antibodies) expression and increased BORIS/CTCF (show CTCF Antibodies) ratio indicate frequent disruption of CTCF (show CTCF Antibodies) and its effector genes in PCa (show FLVCR1 Antibodies).

Mouse (Murine) CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL) interaction partners

  1. The TGFB (show TGFB1 Antibodies) pathway as most affected by embryonic Ctcfl expression.

  2. down-regulation of endogenous BORIS by specific shRNAs inhibited both RNA transcription and cell cycle progression. The results altogether suggest a role for BORIS in coordinating S phase events with mitosis.

  3. Rb2 (show RBL2 Antibodies) coimmunolocalizes with the chromatin insulator CCCTC-binding factor (CTCF (show CTCF Antibodies)) and BORIS in T-antigen-positive but not in T-antigen-negative cells.

  4. These findings show that BORIS expression is more widespread than previously believed, and suggest a role for BORIS in nucleolar function.

  5. findings indicate that BORIS-induced expression of TSP50 (show PRSS50 Antibodies) is governed by accessibility and binding of BORIS to the promoter

  6. These findings define transcriptional regulation of CST (Gal3st1 (show GAL3ST1 Antibodies)) expression as a critical role for BORIS in spermatogenesis.

  7. CTCFL and PRMT7 (show PRMT7 Antibodies) may play a role in male germline imprinted gene methylation.

  8. CTCFL/BORIS is a methylation-independent DNA-binding protein (show HSF4 Antibodies) that preferentially binds to the paternal H19 (show NCKAP1 Antibodies) differentially methylated region

  9. DNA damage-induced and ATR (show ATR Antibodies)/Rfx1 (show RFX1 Antibodies)-mediated increase of miR (show MLXIP Antibodies)-709 expression in exposed testes may be a protective mechanism that effectively decreases a cellular level of BORIS to prevent massive aberrant erasure of DNA methylation (show HELLS Antibodies) after radiation exposure.

CTCFL Antigen Profile

Protein Summary

CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene names and symbols associated with CTCFL

  • CCCTC-binding factor like (CTCFL) antibody
  • CCCTC-binding factor (zinc finger protein)-like (Ctcfl) antibody
  • CCCTC-binding factor like (Ctcfl) antibody
  • Boris antibody
  • CT27 antibody
  • CTCF-T antibody
  • CTCFL antibody
  • dJ579F20.2 antibody
  • HMGB1L1 antibody
  • OTTMUSG00000016680 antibody

Protein level used designations for CTCFL

BORIS-like protein , CTCF paralog , CTCF-like protein , HMG-1L1 , brother of the regulator of imprinted sites , cancer/testis antigen 27 , putative high mobility group protein 1-like 1 , putative high mobility group protein B1-like 1 , transcriptional repressor CTCFL , zinc finger protein CTCF-T , CCCTC-binding factor (zinc finger protein)-like , CCCTC-binding factor-like protein , BORIS , Brother of the regulator of imprinted sites , likely orthologue of H. sapiens CCCTC-binding factor-like protein (CTCFL) , brother of regulator of imprinted sites , transcriptional repressor CTCFL-like

GENE ID SPECIES
140690 Homo sapiens
458360 Pan troglodytes
477271 Canis lupus familiaris
664799 Mus musculus
100079649 Ornithorhynchus anatinus
100144618 Bos taurus
100360757 Rattus norvegicus
100475700 Ailuropoda melanoleuca
701500 Macaca mulatta
100126237 Sus scrofa
100435120 Pongo abelii
100583303 Nomascus leucogenys
100618914 Monodelphis domestica
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