anti-Histone Cluster 1, H3a (HIST1H3A) Antibodies

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Additionally we are shipping Histone Cluster 1, H3a Kits (1) and and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
HIST1H3A 8350 P68431
HIST1H3A 360198 P68433
HIST1H3A    
How to order from antibodies-online
  • +1 877 302 8632
  • +1 888 205 9894 (toll-free)
  • Order online
  • orders@antibodies-online.com

Top anti-Histone Cluster 1, H3a Antibodies at antibodies-online.com

Showing 10 out of 201 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Delivery Price Details
Human Rabbit Un-conjugated WB Host: Rabbit Target Name: HIST1H3A Sample Type: Human 721_B Antibody Dilution: 1.0ug/ml  HIST1H3A is supported by BioGPS gene expression data to be expressed in 721_B WB Suggested Anti-HIST1H3A Antibody Titration: 1.0 ug/ml Positive Control: Jurkat Whole Cell 100 μL 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated IF, WB Western blot analysis of extract from HeLa cells using Histone H3.1 Antibody (S10) (ABIN658963, Lane 1 and 2) and Phospho-Histone H3.1-S10 Antibody (ABIN658964, Lane 3 and 4). Dilution: 1:500~1:1000 Immunofluorescence staining of methanol-fixed HeLa cells using Histone H3.1 Antibody (S10) (ABIN658963, Red). Dilution: 1:100~200 0.1 mg 10 to 11 Days
$534.64
Details
Human Rabbit Un-conjugated IF, IHC, WB Western blot analysis of extract from HeLa cells using Histone H3.1 Antibody (S10) (ABIN658963, Lane 1 and 2) and Phospho-Histone H3.1-S10 Antibody (ABIN658964, Lane 3 and 4). Dilution: 1:500~1:1000 Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-Histone H3.1-S10 Antibody (ABIN658964)(left) or the same antibody preincubated with blocking peptide(right). Dilution: 1:50~1:100 0.1 mg 10 to 11 Days
$534.64
Details
Human Mouse Un-conjugated ChIP, IF, WB Western Blot (Cell lysate) analysis of (1) 25 ug whole cell extracts of HeLa cells, (2) 1 ug of recombinant histone H2A, (3) 1 ug of recombinant histone H2B, (4) 1 ug of recombinant histone H3, and (5) 1 ug of recombinant histone H4. Western Blot analysis of (1) 30 ug whole cell extracts of HeLa cells, (2) 30 ug whole cell extracts of K562 cells, (3) 30 ug whole cell extracts of MCF7 cells, (4) 30 ug whole cell extracts of U2OS cells, (5) 30 ug whole cell extracts of HepG2 cells, (6) 30 ug whole cell extracts of Jurkat cells, (7) 30 ug whole cell extracts of NIH3T3 cells, (8) 30 ug whole cell extracts of E14Tg2a mouse ES cells. 50 μg 11 to 12 Days
$508.50
Details
Human Mouse Un-conjugated ChIPSeq, ChIP, ELISA, IF, WB ChIP assays were performed using human HeLa cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers specific for the promoter of the constitutively expressed GAPDH and c-fos genes, used as positive controls, and for exon 2 of the inactive myoglobin (MB) gene and the Sat2 satellite repeat, used as negative controls. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that trimethylation of K4 at histone H3 is associated with the promoters of active genes. ChIP was performed on sheared chromatin from 1 million HeLaS3 cells using antibody. The figure shows the peak distribution along two 5 Mb regions of chromosome 3 and 5 and in two 100 kb regions surrounding the GAPDH and c-fos positive control genes. These results clearly show an enrichment of the H3K4 trimethylation at the promoters of active genes. 50 μg 11 to 12 Days
$637.32
Details
Human Mouse Un-conjugated ChIP, ELISA, IF, WB ChIP assays were performed using HeLa cells. A titration of the antibody consisting of 1, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for exon 2 of the myoglobin gene. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Western Blot (Cell lysate) analysis of 15 ug histone extracts of HeLa cells. 50 μg 11 to 12 Days
$637.32
Details
Human Mouse Un-conjugated ChIP, ELISA, IF, WB Immunofluorescent staining of Hela cell line with antibody followed by an anti-mouse antibody conjugated to Alexa594 (left). The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings (right). ChIP assays were performed using HeLa cells. A titration of the antibody consisting of 1, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). 50 μg 11 to 12 Days
$637.32
Details
Human Mouse Un-conjugated ChIP, ELISA, IF, WB ELISA is a quantitative method used to determine the titer of the antibody using a serial dilution of antibody against Histone H3 (K9me2). The wells were coated with peptides containing the unmodified H3K9 as well as the monomethylated, dimethylated and trimethylated H3K9 and the dimethylated H3K4 and H3K27. The figure shows a high specificity of the antibody for the modification of interest. Western Blot (Cell lysate) analysis of 15 ug histone extracts of HeLa cells. 50 μg 11 to 12 Days
$637.32
Details
Human Mouse Un-conjugated ChIP, ELISA, IF, WB Immunofluorescent staining of Hela cell line with antibody followed by an anti-mouse antibody conjugated to Alexa594 (left). The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings (right). ChIP assays were performed using HeLa cells. A titration of the antibody consisting of 1, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the promoter and the coding region of the GAPDH gene, and for the RPL10 and HBB promoters. The figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). 50 μg 11 to 12 Days
$637.32
Details
Human Rabbit Un-conjugated ChIP, ELISA, ICC, IHC (p), WB ChIP analysis of HeLa cells with Histone H3 (acetyl K9) monoclonal antibody, clone RM161  under 5 ug working concentration. Real-time PCR was performed using primers specific to the gene indicated. Sandwich ELISA analysis of HeLa cells treated or untreated with sodium butyrate using Histone H3 (acetyl K9) monoclonal antibody, clone RM161  as the detection antibody under 1 ug/mL working concentration. Using biotinylated format of Pan Histone H3 monoclonal antibody, clone RM188  as the capture antibody under 1 ug/mL working concentration. 100 μg 11 to 12 Days
$521.33
Details

More Antibodies against Histone Cluster 1, H3a Interaction Partners

Human Histone Cluster 1, H3a (HIST1H3A) interaction partners

  1. the H3 K27M mutation was mainly associated with a poorer prognosis in infratentorial gliomas compared with the corresponding H3 wild-type gliomas

  2. In 2 cases, we identified H3F3A mutation, classically described in pediatric midline gliomas

  3. Data indicate the mechanism for epigenetic regulation in cancer by inducing E3 ubiquitin ligase NEDD4-dependent histone H3 ubiquitination.

  4. the identification of increased expression of H3K27me3 during a patient's clinical course can be helpful for determining whether the tumors are heterochronous

  5. Here, we report that JMJD5, a Jumonji C (JmjC) domain-containing protein, is a Cathepsin L-type protease that mediates histone H3 N-tail proteolytic cleavage under stress conditions that cause a DNA damage response.

  6. Data suggest that Ki-67 antigen proliferative index has important limitations and hhosphohistone H3 (PHH3) is an alternative proliferative marker.

  7. These results identify cytokine-induced histone 3 lysine 27 trimethylation as a mechanism that stabilizes gene silencing in macrophages

  8. This data indicates that, in the early developing human brain, HIST1H3B constitutes the largest proportion of H3.1 transcripts among H3.1 isoforms.

  9. This series of 47 diffuse midline gliomas, histone H3-K27M mutation was mutually exclusive with IDH1-R132H mutation and EGFR amplification, rarely co-occurred with BRAF-V600E mutation, and was commonly associated with p53 overexpression, ATRX loss, and monosomy 10. Among these K27M+ diffuse midline gliomas.

  10. Data show that histone chaperone HIRA co-localizes with viral genomes, binds to incoming viral and deposits histone H3.3 onto these.

  11. These experiments showed that PHF13 binds specifically to DNA and to two types of histone H3 methyl tags (lysine 4-tri-methyl or lysine 4-di-methyl) where it functions as a transcriptional co-regulator.

  12. Hemi-methylated CpGs DNA recognition activates UHRF1 ubiquitylation towards multiple lysines on the H3 tail adjacent to the UHRF1 histone-binding site.

  13. We describe, for the first time, the MR imaging features of pediatric diffuse midline gliomas with histone H3 K27M mutation

  14. Approximately 30% of pediatric high grade gliomas (pedHGG) including GBM and DIPG harbor a lysine 27 mutation (K27M) in histone 3.3 (H3.3) which is correlated with poor outcome and was shown to influence EZH2 function.

  15. H3F3A K27M mutation in adult cerebellar HGG is not rare.

  16. Data show that lysyl oxidase-like 2 (LOXL2) is a histone modifier enzyme that removes trimethylated lysine 4 (K4) in histone H3 (H3K4me3) through an amino-oxidase reaction.

  17. Histone H3 lysine 9 (H3K9) acetylation was most prevalent when the Dbf4 transcription level was highest whereas the H3K9me3 level was greatest during and just after replication.

  18. SPOP-containing complex regulates SETD2 stability and H3K36me3-coupled alternative splicing.

  19. Data suggest that binding of helical tail of histone 3 (H3) with PHD ('plant homeodomain') fingers of BAZ2A or BAZ2B (bromodomain adjacent to zinc finger domain 2A or 2B) requires molecular recognition of secondary structure motifs within H3 tail and could represent an additional layer of regulation in epigenetic processes.

  20. The results demonstrate a novel mechanism by which Kdm4d regulates DNA replication by reducing the H3K9me3 level to facilitate formation of preinitiation complex.

Mouse (Murine) Histone Cluster 1, H3a (HIST1H3A) interaction partners

  1. Hemi-methylated DNA opens a closed conformation of UHRF1 to facilitate its H3 histone recognition.

  2. Findings show that CCL2 and CCL3 are upregulated in the injured peripheral nerve through epigenetic histone modification in infiltrating immune cells such as macrophages.

  3. This result suggests that the incorporation of H3.1 has a detrimental effect on the process of genome remodeling and contributes to the low success rate of somatic nuclear cloning.

Histone Cluster 1, H3a (HIST1H3A) Antigen Profile

Protein Summary

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails\; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Gene names and symbols associated with HIST1H3A

  • histone cluster 1 H3 family member a (HIST1H3A) antibody
  • histone cluster 1, H3a (Hist1h3a) antibody
  • H3/A antibody
  • H3FA antibody

Protein level used designations for HIST1H3A

H3 histone family, member A , histone 1, H3a , histone H3.1 , histone H3/a

GENE ID SPECIES
8350 Homo sapiens
360198 Mus musculus
Selected quality suppliers for anti-Histone Cluster 1, H3a (HIST1H3A) Antibodies
Did you look for something else?