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HIST1H3A antibody (acLys27)

Name: HIST1H3A antibody (acLys27)
Brand: antibodies-online
SKU: ABIN7139175
Price: 350.9 USD
Availability: InStock

HIST1H3A Reactivity: Human ELISA, WB, IF, ChIP, ICC, IP Host: Rabbit Polyclonal unconjugated

Western Blot Detected samples: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, Untreated (-) or treated (+) with 30 mM sodium butyrate for 4h All lanes: HIST1H3A antibody at 1:100 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa (HIST1H3A antibody (acLys27))

Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with ABIN7139175 at 1:5, counter-stained with DAPI. (HIST1H3A antibody (acLys27))

Immunocytochemistry analysis of ABIN7139175 diluted at 1:10 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. (HIST1H3A antibody (acLys27))

ICC

Chromatin Immunoprecipitation Hela (4*10 6 , treated with 30 mM sodium butyrate for 4h) were treated with Benzanase, sonicated, and immunoprecipitated with 5 μg anti-HIST1H3A (ABIN7139175) or a control normal rabbit IgG. (HIST1H3A antibody (acLys27))

IHC

Immunoprecipitating HIST1H3A in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of ABIN7139175 in HepG2 whole cell lysate. (HIST1H3A antibody (acLys27))

5 images

Catalog No. ABIN7139175

Datasheet as PDF

Target See all HIST1H3A Antibodies

HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))
Binding Specificity
16

8

6

6

6

6

5

5

5

5

5

5

4

4

4

4

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2
acLys27
Reactivity
242

67

50

16

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1
Human
Host
235

6

1
Rabbit
Compatible Secondaries
Clonality
176

66
Polyclonal
Conjugate
223

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1
This HIST1H3A antibody is un-conjugated
Application
202

182

72

68

61

57

52

27

19

17

16

13

13

7

5
ELISA, Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Cross-Reactivity

Human

Purification

Antigen Affinity Purified

Immunogen

Peptide sequence around site of Acetyl-Lys (27) derived from Human Histone H3.1

Isotype

IgG

Top Product

Discover our top product HIST1H3A Primary Antibody
anti-Histone Cluster 1, H3a (HIST1H3A) (pSer10) antibody
HIST1H3A Reactivity: Human ELISA, WB, IF, ChIP, DB Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H3a (HIST1H3A) (3meLys27), (pSer28) antibody
HIST1H3A Reactivity: Human ELISA, WB, ChIP, DB, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H3a (HIST1H3A) (2meArg17 (asymetric)), (acLys18) antibody
HIST1H3A Reactivity: Human, Mouse ELISA, WB, IF, ChIP, DB, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H3a (HIST1H3A) (acLys4) antibody
HIST1H3A Reactivity: Human ELISA, WB, IF, ChIP, DB, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H3a (HIST1H3A) (acLys18) antibody
HIST1H3A Reactivity: Human, Mouse ELISA, WB, IF, ChIP, DB, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H3a (HIST1H3A) antibody
HIST1H3A Reactivity: Human WB, IHC, IF, ChIP Host: Rabbit Monoclonal unconjugated

anti-Histone Cluster 1, H3a (HIST1H3A) (meLys4) antibody
HIST1H3A Reactivity: Human, Mouse ELISA, WB, IF, ChIP, DB, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H3a (HIST1H3A) (3meLys9) antibody
HIST1H3A Reactivity: Human, Mouse ELISA, WB, IF, ChIP, DB, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H3a (HIST1H3A) (3meLys27) antibody
HIST1H3A Reactivity: Human, Mouse, Arabidopsis, Poplar (Populus), Tomato, Zea mays, Drosophila melanogaster, Nematode ELISA, WB, IF, ChIP, DB, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone Cluster 1, H3a (HIST1H3A) (3meLys36) antibody
HIST1H3A Reactivity: Human, Mouse, Arabidopsis, Oryza sativa ELISA, WB, IF, ChIP, DB, ChIP-seq Host: Rabbit Polyclonal unconjugated

Application Notes

Recommended dilution: WB:1:100-1:1000, ICC:1:10-1:100, IF:1:1-1:10, IP:1:200-1:2000,

Restrictions

For Research Use only
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C,-80 °C

Storage Comment

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

HIST1H3A (Histone Cluster 1, H3a (HIST1H3A))

Alternative Name

HIST1H3A (HIST1H3A Products)

Synonyms

H3/A antibody, H3FA antibody, histone cluster 1 H3 family member a antibody, histone cluster 1, H3a antibody, HIST1H3A antibody, Hist1h3a antibody

Background

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Aliases: H3 histone family member E pseudogene antibody, H3 histone family, member A antibody, H3/A antibody, H31_HUMAN antibody, H3F3 antibody, H3FA antibody, Hist1h3a antibody, HIST1H3B antibody, HIST1H3C antibody, HIST1H3D antibody, HIST1H3E antibody, HIST1H3F antibody, HIST1H3G antibody, HIST1H3H antibody, HIST1H3I antibody, HIST1H3J antibody, HIST3H3 antibody, histone 1, H3a antibody, Histone cluster 1, H3a antibody, Histone H3 3 pseudogene antibody, Histone H3.1 antibody, Histone H3/a antibody, Histone H3/b antibody, Histone H3/c antibody, Histone H3/d antibody, Histone H3/f antibody, Histone H3/h antibody, Histone H3/i antibody, Histone H3/j antibody, Histone H3/k antibody, Histone H3/l antibody

UniProt

P68431