anti-Keratin 8 (KRT8) Antibodies

KRT8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Additionally we are shipping Keratin 8 Proteins (44) and Keratin 8 Kits (21) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
KRT8 3856 P05787
KRT8 16691 P11679
KRT8 25626 Q10758
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Top anti-Keratin 8 Antibodies at antibodies-online.com

Showing 10 out of 970 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated IHC, WB WB Suggested Anti-KRT8 Antibody Titration:  0.2-1 ug/ml  Positive Control:  Jurkat cell lysate Rabbit Anti-KRT8 Antibody  Paraffin Embedded Tissue: Human Kidney Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0-8.0 ug/ml Magnification: 400X 100 μL Log in to see 2 to 3 Days
$289.00
Details
Cow Rabbit Un-conjugated IHC, WB WB Suggested Anti-KRT8 Antibody Titration:  0.2-1 ug/ml  Positive Control:  HepG2 cell lysate KRT8 is supported by BioGPS gene expression data to be expressed in HepG2 Immunohistochemistry with Small intestine tissue at an antibody concentration of 5µg/ml using anti-KRT8 antibody (ARP42029_P050) 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated ICC, IF, IHC, IHC (p), WB Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-16094] - Immunohistochemical analysis of paraffin-embedded Gastric ca, using antibody at 1:500 dilution. Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Sample (30 ug of whole cell lysate) A: JC 10% SDS PAGE gel, diluted at 1:10000. 0.1 mL Log in to see 7 to 9 Days
$559.35
Details
Human Rabbit Un-conjugated IHC (p), WB Western blot analysis of Cytokeratin 8 using anti- Cytokeratin 8 antibody .  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.   Lane 1: rat liver tissue lysates,   Lane 2: mouse ovary tissue lysates,   Lane 3: mouse liver tissue lysates,   Lane 4: MCF-7 whole cell lysates,  Lane 5: A549 whole cell lysates,  Lane 6: HELA whole Cell lysates.  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Cytokeratin 8 antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 8 at approximately 54KD, 56KD. The expected band size for Cytokeratin 8 is at 54KD. IHC analysis of Cytokeratin 8 using anti- Cytokeratin 8 antibody . Cytokeratin 8 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti- Cytokeratin 8 Antibody  overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg Log in to see 4 to 6 Days
$280.00
Details
Human Mouse Un-conjugated FACS, IHC (fro), IF, IHC (p), WB   0.1 mg Log in to see 6 to 8 Days
$434.50
Details
Human Mouse Un-conjugated ELISA, ICC, IHC, WB Figure 1: Western blot analysis using CK8 mouse mAb against A549 (1), Hela (2), MCF-7 (3), A431 (4) and HepG2 (5) cell lysate. Dilution: 1/500 - 1/2000 Figure 2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma (A), lung cancer (B) and ovarian cancer tissue (C), showing membrane and cytoplasmic localization with DAB staining using CK8 mouse mAb. Dilution: 1/200 - 1/1000 100 μL Log in to see 10 to 11 Days
$577.50
Details
Human Rabbit Un-conjugated ELISA, IHC (p), WB Immunohistochemistry (IHC) analysis of paraffin-embedded Human Kidney, antibody was diluted at 1:100. 100 μL Log in to see Available
$181.73
Details
Cow Rabbit Un-conjugated IC, IF, IHC, WB Immunofluorescent analysis of Cytokeratin 8 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Immunohistochemical analysis of Cytokeratin 8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 200 μL Log in to see 13 to 14 Days
$487.50
Details
Human Rabbit Un-conjugated IC, IF, IHC, WB Immunofluorescent analysis of Cytokeratin 8 staining in DLD cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Immunohistochemical analysis of Cytokeratin 8 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 200 μL Log in to see 13 to 14 Days
$487.50
Details
Human Rabbit Un-conjugated IC, IF, IHC, WB Immunofluorescent analysis of Cytokeratin 8 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Immunohistochemical analysis of Cytokeratin 8 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. 200 μL Log in to see 13 to 14 Days
$487.50
Details

Top referenced anti-Keratin 8 Antibodies

  1. Human Monoclonal KRT8 Primary Antibody for ICC, FACS - ABIN335327 : Ivanyi, Groeneveld, Van Doornewaard, Mooi, Hageman: Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis. in Cancer research 1990 (PubMed)
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  2. Human Monoclonal KRT8 Primary Antibody for ICC, IHC (fro) - ABIN920530 : Troyanovsky, Guelstein, Tchipysheva, Krutovskikh, Bannikov: Patterns of expression of keratin 17 in human epithelia: dependency on cell position. in Journal of cell science 1990 (PubMed)
    Show all 4 Pubmed References

  3. Hamster Monoclonal KRT8 Primary Antibody for IHC (fro), IHC (p) - ABIN920532 : Moll, Franke, Schiller, Geiger, Krepler: The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. in Cell 1983 (PubMed)
    Show all 4 Pubmed References

  4. Human Polyclonal KRT8 Primary Antibody for IF (cc), IF (p) - ABIN737751 : Zhang, Li, Fu, Li: Calcitonin is expressed in the submaxillary glands of rats. in Bosnian journal of basic medical sciences / Udruženje basi?nih mediciniskih znanosti = Association of Basic Medical Sciences 2014 (PubMed)
    Show all 3 Pubmed References

  5. Human Polyclonal KRT8 Primary Antibody for ICC, IF - ABIN4303529 : Wu, Wang, Liu, Bai, Li, Ji, Peng, Yu, Wang: MiR-195 participates in the placental disorder of preeclampsia via targeting activin receptor type-2B in trophoblastic cells. in Journal of hypertension 2016 (PubMed)
    Show all 3 Pubmed References

  6. Human Monoclonal KRT8 Primary Antibody for IF, IHC - ABIN965965 : Walker, Harris, Holloway, McKenzie, Wells, Robinson, Morris: Cytokeratin KRT8/18 expression differentiates distinct subtypes of grade 3 invasive ductal carcinoma of the breast. in Cancer genetics and cytogenetics 2007 (PubMed)
    Show all 2 Pubmed References

  7. Human Monoclonal KRT8 Primary Antibody for IHC (fro), IHC (p) - ABIN284055 : Fernandez-Valdivia, Mukherjee, Ying, Li, Paquet, DeMayo, Lydon: The RANKL signaling axis is sufficient to elicit ductal side-branching and alveologenesis in the mammary gland of the virgin mouse. in Developmental biology 2009 (PubMed)
    Show all 2 Pubmed References

  8. Human Monoclonal KRT8 Primary Antibody for IHC (fro), IHC (p) - ABIN284054 : Bardag-Gorce, Oliva, Li, French, French: SAMe prevents the induction of the immunoproteasome and preserves the 26S proteasome in the DDC-induced MDB mouse model. in Experimental and molecular pathology 2010 (PubMed)
    Show all 2 Pubmed References

  9. Chicken Monoclonal KRT8 Primary Antibody for IHC (fro), IHC (p) - ABIN268153 : Rossdeutscher, Li, Luco, Fadhil, Ochietti, Camirand, Huang, Reinhardt, Muller, Kremer: Chemoprevention activity of 25-hydroxyvitamin D in the MMTV-PyMT mouse model of breast cancer. in Cancer prevention research (Philadelphia, Pa.) 2015 (PubMed)
    Show all 2 Pubmed References

  10. Human Monoclonal KRT8 Primary Antibody for ICC, IHC (p) - ABIN94298 : Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. in The Journal of pathology 1991 (PubMed)

More Antibodies against Keratin 8 Interaction Partners

Human Keratin 8 (KRT8) interaction partners

  1. We found close association between changes in K8/K18 expression and cell death/cell survival events in the human granulosa cell lineage. Large secondary and early antral follicles and regressing corpora lutea showed low/absent K8/K18 expression. Conversely, early growing antral follicles, functional menstrual corpora lutea, as well as corpus luteum of pregnancy, where cell death was scarce, showed high K8/K18 expression.

  2. Cytokeratin 5/6 and CK8/18 immunohistochemistry on 150 cases of triple negative breast cancers was performed and association with various clinicopathological features was evaluated.

  3. KRT8 mRNA and protein are upregulated in anaplastic thyroid carcinoma. KRT8 protein binds to annexin A2.

  4. K8/K18 variants are overrepresented in Chinese NAFLD patients and might accelerate liver fat storage through insulin resistance.

  5. regulated enhancement of autophagy with concurrent increased expression of KRT8 and the inhibition of KRT8 phosphorylation serve to inhibit oxidative stress-induced epithelial-mesenchymal transitionof retinal pigment epithelium cells.

  6. Histopathological differences between Magnetic resonance-guided high-intensity focused ultrasound (MR-HIFU)-ablated tissue and radiofrequency (RF)-ablated tissue were demonstrated. CK-8 was more reliable for cell viability assessment than H&E when used directly after ablation

  7. Loss of activating transcription factor 3 (ATF3) in knockout mice promotes the emergence of keratins CK5+CK8+ epithelial cells.

  8. notochord-specific marker during early intervertebral disc development

  9. Data show that the filament elongation of both desmin and keratin K8/K18 proceeds very similar to that of vimentin.

  10. New insights into interactions between the nucleotide-binding domain of CFTR and keratin 8.

  11. We present results of a multicentre study measuring UBC((R)) Rapid Test(test that detects fragments of cytokeratins 8 and 18 in urine) in bladder cancer patients and healthy controls with focus on carcinoma in situ (CIS) and high-grade bladder cancer.

  12. Data show that after siRNA transfection, TGF-beta1 protein level was decreased, and CK18 proteins was decreased, while CK8 proteins was increased, and TERT protein expression was slightly increased at 96 h.

  13. The interplay between Solo protein and keratin 8/keratin 18 filaments plays a crucial role in tensile force-induced RhoA activation and consequent actin cytoskeletal reinforcement in endothelial cells.

  14. K8/18 filaments provide resistance to apoptosis in granulosa cell tumor cells by impairing FAS expression.

  15. These metastatic tumors revealed no detectable expression of CK8/18, E-cadherin, VCAM-1, and ICAM-1

  16. Data show that loss of epithelial membrane protein 2 (EMP2) is involved in sphingosylphosphorylcholine (SPC)-induced phosphorylation of keratin 8 (K8) via ubiquitination of protein phosphatase 2 (PP2A) through alpha4 phosphoprotein by caveolin-1 (cav-1).

  17. The reactivity of rheumatoid arthritis (RA) serum IgG antibodies against K8 was significantly higher than healthy controls and systemic lupus erythematosus patients, confirming K8 as a novel autoantigen of RA.

  18. Variants in highly conserved residues of K8 Lys393Arg (ezetimibe/simvastatin-related) was found in patients with fatal Drug-induced liver injury.

  19. Keratins 8 and 18 are type II acute-phase responsive genes overexpressed in human liver disease.

  20. In human failing myocardium, where TNF-alpha expression is upregulated, K8/K18 were also ectopically expressed

Mouse (Murine) Keratin 8 (KRT8) interaction partners

  1. The authors found that keratin 8 suppressed toll-like receptor-induced nuclear factor (NF)-kappaB activation and interacted with the adaptor tumor necrosis factor (TNF) receptor-associated factor 6 (TRAF6) to prevent its polyubiquitination.

  2. K8/K18 interact with Notch1 and regulate Notch1 signalling activity during differentiation of the colonic epithelium.

  3. Mitochondria in K8 wild-type beta cells and MIN6 insulinoma cells overexpressing K8 and 18 are more stationary compared with mitochondria in keratin-deficient cells

  4. K8/K18-dependent PKCdelta- and ASMase-mediated modulation of lipid raft size can explain the more prominent FasR-mediated signaling resulting from K8/K18 loss.

  5. DRA mRNA levels were decreased by three- to fourfold and DRA protein was almost entirely lost in K8-/- cecum and proximal and distal colon compared with K8+/+.

  6. Upregulation of IL-22 in combination with a complete loss of its negative regulator IL-22BP, and increased downstream STAT3-signaling in K8(-/-) and K8(-/-)Apc(Min/+) colonic epithelia confirmed that the IL-22 pathway, important in inflammation, proliferation and tissue regeneration

  7. Krt8 deletion (Krt8(-/-)) in a mouse model of cystic fibrosis (F508del-Cftr mice) increased the levels of circulating markers of bone formation, corrected the expression of osteoblast phenotypic genes, promoted trabecular bone formation and improved bone mass and microarchitecture. Krt8 deletion corrected overactive NF-kappaB signaling & decreased Wnt-beta-catenin signaling induced by F508del-Cftr mutation in osteoblasts.

  8. Autoantibodies to several antigens were identified in 81% of K8-null male mice 8 mo or older. Similar autoantibodies were detected in aging K18-null male mice that had a related liver phenotype but normal colon compared with K8-null mice

  9. The regulation of the SCFA-MCT1-HMGCS2 axis is disrupted in K8(-/-) colonocytes, suggesting a role for keratins in colonocyte energy metabolism and homeostasis.

  10. using knockout mice, we show K8+/- colon expresses ~50% less keratins compared to K8 wild type. In experimental colitis, K8+/- mice showed higher disease sensitivity and delayed recovery compared to K8+/+ littermates.

  11. In several liver stress models epiplakin and K8 genes displayed identical expression patterns and transgenic K8 overexpression resulted in elevated hepatic epiplakin levels.

  12. In human failing myocardium, where TNF-alpha expression is upregulated, K8/K18 were also ectopically expressed

  13. The findings demonstrate the near complete loss of K8/K18 with concomitant high levels of vimentin in CT26 cells, a chemically-induced mouse colonic tumor.

  14. Directed expression of a chimeric type II keratin partially rescues keratin 5-null mice.

  15. Our data demonstrate that HF diet triggers aggregate formation and development of liver injury in susceptible individuals through misfolding and crosslinking of excess K8.

  16. K8 inactivation leads to reduced fasting blood glucose levels and resistance to hyperglycaemia after glucose challenge.

  17. Keratin 8 is required for the maintenance of architectural structure in thymus epithelium.

  18. A conserved rod domain phosphotyrosine that is targeted by the phosphatase PTP1B promotes keratin 8 protein insolubility and filament organization.

  19. K8 acetylation at Lys-207, a highly conserved residue among type II keratins, is up-regulated upon hyperglycemia and down-regulated by SIRT2.

  20. Data indicate that cyclin D1 is expressed exclusively in luminal keratin 8 immunoreactive tumor cells and is dependent on the adipose secreted hormone leptin.

Cow (Bovine) Keratin 8 (KRT8) interaction partners

  1. Results did not support that K8/K18 filaments influence the expression of Fas on the surface of luteal cells.

Zebrafish Keratin 8 (KRT8) interaction partners

  1. Data identify Danio rerio keratins 8/18 as the true orthologs of the human keratin pair 8/18.

Pig (Porcine) Keratin 8 (KRT8) interaction partners

  1. This study reveals clear genetic-statistical evidence for a link of KRT8, FAF1 and PTH1R with some of leg weakness related traits in pigs.

  2. Mechanical injury of vascular smooth muscle up-regulated keratin 8.

Keratin 8 (KRT8) Antigen Profile

Protein Summary

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.

Gene names and symbols associated with anti-Keratin 8 (KRT8) Antibodies

  • keratin 8 (KRT8) antibody
  • keratin 8 (Krt8) antibody
  • keratin 8 S homeolog (krt8.S) antibody
  • keratin 8 (krt8) antibody
  • AA960620 antibody
  • AL022697 antibody
  • AU019895 antibody
  • card2 antibody
  • cb186 antibody
  • CK-8 antibody
  • ck8 antibody
  • cyk8 antibody
  • CYKER antibody
  • DKFZp468F2127 antibody
  • DreK8 antibody
  • EndoA antibody
  • k2c8 antibody
  • K8 antibody
  • KERATIN8 antibody
  • KO antibody
  • Krt-2.8 antibody
  • krt2-5 antibody
  • krt2-8 antibody
  • KRT8 antibody
  • MGC69490 antibody
  • sb:cb186 antibody
  • wu:fa20h05 antibody
  • wu:fa95h10 antibody
  • wu:fb96c06 antibody
  • zf-K8 antibody
  • zfk8 antibody

Protein level used designations for anti-Keratin 8 (KRT8) Antibodies

cytokeratin 8 , cytokeratin-8 , keratin, type II cytoskeletal 8 , type-II keratin Kb8 , CK-8 , cytokeratin endo A , keratin complex 2, basic, gene 8 , keratin-8 , K8 , KERA , cytokeratin-A , keratin 8 , keratin complex 2, basic, gene 5 , etID33550.23 , etID43142.23 , etID43142.8 , keratin, type 2, gene 8

GENE ID SPECIES
3856 Homo sapiens
16691 Mus musculus
25626 Rattus norvegicus
281269 Bos taurus
380217 Xenopus laevis
426896 Gallus gallus
431676 Xenopus (Silurana) tropicalis
486513 Canis lupus familiaris
700449 Macaca mulatta
737628 Pan troglodytes
797433 Danio rerio
100152077 Sus scrofa
100174430 Pongo abelii
101110495 Ovis aries
100348891 Oryctolagus cuniculus
100736243 Cavia porcellus
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