Keratin 8 (KRT8) antibody

Details for Product No. ABIN94300
Antigen
Reactivity
Human, Pig (Porcine), Cow (Bovine), Sheep (Ovine), Rabbit
738
169
156
87
78
72
70
36
34
12
8
5
3
2
2
2
1
1
1
1
Host
Mouse
472
277
5
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
35
19
9
9
9
9
9
9
9
9
9
9
9
9
9
8
8
8
8
8
8
6
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
413
357
297
270
202
139
130
63
55
48
28
26
22
14
13
6
2
2
1
1
Options
Immunogen Cytoskeleton preparation from HeLa human cervix carcinoma cell line.
Clone C-43
Isotype IgG1
Specificity The antibody C-43 reacts with Cytokeratin 8 (52.5 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
No Cross-Reactivity Mouse (Murine), Rat (Rattus), Hamster, Chicken, Xenopus laevis
Cross-Reactivity (Details) Sheep, Human, Bovine, Rabbit, Porcine
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name Cytokeratin 8 (KRT8 Antibody Abstract)
Background Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Keratin 8 (KRT8) antibody (ABIN94300) Detection of cytokeratin 8 in HeLa cell lysate (reducing conditions) by mouse monoclo...
Product cited in: Bártek, Vojt?sek, Stasková, Bártková, Kerekés, Rejthar, Kovarík: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).