Nidogen 2 Proteins (NID2)

Mouse (Murine) Nidogen 2 (NID2) interaction partners

1. Nidogen-2, a protein shown to be expressed intracellularly and for secretion by pre-adipocytes, was shown to interact, through its globular G3 domain, with TRAP 5a in vitro.

2. finding demonstrates that nidogens, although homologous proteins, exert their effect on BM assembly through different binding domains, which may in turn result in alterations of BM structure and functions

3. show that loss of nidogen 2, but not of nidogen 1, promotes lung metastasis of melanoma cells. Histological and ultrastructural analysis of nidogen 1- and 2-deficient lungs did not reveal differences in morphology and ultrastructure of basement membrane

4. Basement membrane deposition of nidogen 1 but not nidogen 2 requires the nidogen binding module of the laminin gamma1 chain.

5. These findings revealed extensive coregulation of nidogen-1 and -2 expression and much more complementary functions of the two nidogens than previously recognized.

6. Nid2 is essential for late stages of lung development and for maintenance and/or integrity of cardiac tissue

7. nidogen-2 is equivalent to nidogen-1 in skin-organotypic coculture, and both can promote the development of a functional basement membrane zone

8. nidogen-1 and -2 have roles in development of the ectodermal basement membrane of the limb bud

9. decreased expression of the entactin-2 gene after treatment of the cells with ent-2-specific siRNA preparation led to reduced expression of the cell cycle inhibitor p21

10. The lack of nidogen-1, nidogen-2, or both nidogens, plays no crucial role in the occurrence and localization of laminin-111, collagen type IV, and perlecan in murine tubular renal basement membranes.

11. In capillaries, basement membrane formation is severely impaired in the absence of nidogen-1 and -2, showing an irregular, patchy distribution and a dramatically reduced deposition of collagen IV, perlecan, and particularly laminin-411.

12. Neuromuscular junctions in mutant mice lacking nidogen-2 appear normal at birth, but become topologically abnormal as they mature

Human Nidogen 2 (NID2) interaction partners

1. High NID2 methylation is associated with non-small cell lung cancer.

2. the serum NID2 levels show significant elevation in the ESCC patients and those with relatively high serum NID2 levels ( 32.6 mu mu g/L) are significantly associated with poor survival outcome compared with those with very low serum NID2 levels (< < 20.0 mu mu g/L).

3. Addition of a TWIST1/NID2-based DNA methylation assay adds diagnostic value to urine cytology and the model is sensitive to the classification of equivocal cytology.

4. Promoter methylation of NID2 was significantly higher in nasopharyngeal carcinoma and esophageal squamous cell carcinoma cells. NID2 suppresses the EGFR/Akt and integrin/FAK/PLCgamma metastasis-related pathways.

5. Knockdown of nidogen-2 reduced SOX9 expression, whereas it up-regulated RUNX2 expression

6. The cleavage of nidogen-1 and -2 is partially inhibited in human tumor samples.

7. combined methylation analysis of Twist homolog 1 and nidogen-2 genes may be a simple, noninvasive, sensitive, and specific method for detecting cancer cells in urine.

8. Decreased nidogen-2 expression is associated with hepatocellular carcinoma

9. NID2 promoter hypermethylation is associated with oral cavity squamous cell carcinoma.

10. Nidogen-2 is a new biomarker for ovarian cancer which correlates closely with CA125.

11. short arm region of laminin-5 gamma2 chain: structure, mechanism of processing and binding to heparin, nidogen-1, fibulin-1 and fibulin-2

12. found to be ubiquitous component of basement membrane zones underneath developing epithelia of most of the major organ systems; may participate in cell biological functions during human development

13. Nidogen-2 compensation hypothesis to explain limited phenotype observed following elimination of nidogen-1 gene.

14. nidogen-2 is equivalent to nidogen-1 in skin-organotypic coculture, and both can promote the development of a functional basement membrane zone

15. CpG islands of both NID1 and NID2 genes are aberrantly methylated in human cancer samples and cancer cell lines.