Nidogen 2 Proteins (NID2)

Mouse (Murine) Nidogen 2 (NID2) interaction partners

1. Nidogen-2, a protein shown to be expressed intracellularly and for secretion by pre-adipocytes, was shown to interact, through its globular G3 domain, with TRAP 5a in vitro.

2. show that loss of nidogen 2, but not of nidogen 1 (show NID1 Proteins), promotes lung metastasis of melanoma cells. Histological and ultrastructural analysis of nidogen 1 (show NID1 Proteins)- and 2-deficient lungs did not reveal differences in morphology and ultrastructure of basement membrane

3. Basement membrane deposition of nidogen 1 but not nidogen 2 requires the nidogen binding module of the laminin gamma1 chain.

4. These findings revealed extensive coregulation of nidogen-1 (show NID1 Proteins) and -2 expression and much more complementary functions of the two nidogens than previously recognized.

5. Nid2 is essential for late stages of lung development and for maintenance and/or integrity of cardiac tissue

6. nidogen-2 is equivalent to nidogen-1 (show NID1 Proteins) in skin-organotypic coculture, and both can promote the development of a functional basement membrane zone

7. nidogen-1 (show NID1 Proteins) and -2 have roles in development of the ectodermal basement membrane of the limb bud

8. decreased expression of the entactin-2 gene after treatment of the cells with ent-2 (show SLC29A2 Proteins)-specific siRNA preparation led to reduced expression of the cell cycle inhibitor p21

9. The lack of nidogen-1 (show NID1 Proteins), nidogen-2, or both nidogens, plays no crucial role in the occurrence and localization of laminin-111, collagen type IV (show COL4 Proteins), and perlecan (show HSPG2 Proteins) in murine tubular renal basement membranes.

10. In capillaries, basement membrane formation is severely impaired in the absence of nidogen-1 (show NID1 Proteins) and -2, showing an irregular, patchy distribution and a dramatically reduced deposition of collagen IV (show COL4 Proteins), perlecan (show HSPG2 Proteins), and particularly laminin-411.

Human Nidogen 2 (NID2) interaction partners

1. High NID2 methylation is associated with non-small cell lung cancer.

2. the serum NID2 levels show significant elevation in the ESCC patients and those with relatively high serum NID2 levels ( 32.6 mu mu g/L) are significantly associated with poor survival outcome compared with those with very low serum NID2 levels (< < 20.0 mu mu g/L).

3. Addition of a TWIST1 (show TWIST1 Proteins)/NID2-based DNA methylation (show HELLS Proteins) assay adds diagnostic value to urine cytology and the model is sensitive to the classification of equivocal cytology.

4. Promoter methylation of NID2 was significantly higher in nasopharyngeal carcinoma and esophageal squamous cell carcinoma cells. NID2 suppresses the EGFR (show EGFR Proteins)/Akt (show AKT1 Proteins) and integrin/FAK (show PTK2 Proteins)/PLCgamma metastasis-related pathways.

5. Knockdown of nidogen-2 reduced SOX9 (show SOX9 Proteins) expression, whereas it up-regulated RUNX2 (show RUNX2 Proteins) expression

6. The cleavage of nidogen-1 (show NID1 Proteins) and -2 is partially inhibited in human tumor samples.

7. combined methylation analysis of Twist homolog 1 (show TWIST1 Proteins) and nidogen-2 genes may be a simple, noninvasive, sensitive, and specific method for detecting cancer cells in urine.

8. Decreased nidogen-2 expression is associated with hepatocellular carcinoma

9. NID2 promoter hypermethylation is associated with oral cavity squamous cell carcinoma.

10. Nidogen-2 is a new biomarker for ovarian cancer which correlates closely with CA125 (show MUC16 Proteins).