anti-Tetraspanin 12 (TSPAN12) Antibodies

The protein encoded by TSPAN12 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Additionally we are shipping TSPAN12 Proteins (3) and and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
TSPAN12 23554 O95859
TSPAN12 269831 Q8BKT6
TSPAN12 362326 Q569A2
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Top anti-TSPAN12 Antibodies at antibodies-online.com

Showing 10 out of 53 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Delivery Price Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-TSPAN12 Antibody Titration:  0.2-1 ug/ml  ELISA Titer:  1:312500  Positive Control:  Human Thymus 100 μL 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated ELISA, IHC, WB Western blot analysis of TSPAN12 using anti-TSPAN12 antibody .  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each  Lane was loaded with 50ug of sample under reducing conditions.   Lane 1: human Hela cell lysates,  Lane 2: human SW620 cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSPAN12 antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSPAN12 at approximately 43KD. The expected band size for TSPAN12 is at 35KD. IHC analysis of TSPAN12 using anti-TSPAN12 antibody . TSPAN12 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-TSPAN12 Antibody  overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. 100 μg 4 to 6 Days
$280.00
Details
Bat Rabbit Un-conjugated WB 100 μL 11 to 14 Days
$551.83
Details
Bat Rabbit Un-conjugated IHC, IHC (p), WB Human Lung, Respiratory Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE) Anti-TSPAN12 antibody IHC staining of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugate ... 100 μL 11 to 14 Days
$727.83
Details
Human Rabbit Un-conjugated FACS, IHC (p), WB Western blot analysis of TSPAN12 Antibody (C-term) (ABIN652637) in T47D cell line lysates (35 µg/lane). TSPAN12 (arrow) was detected using the purified polyclonal antibody. Formalin-fixed and paraffin-embedded human brain tissue reacted with TSPAN12 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. 400 μL 10 to 11 Days
$385.00
Details
Human Rabbit Un-conjugated EIA, WB Western blot analysis of TSPAN12 Antibody (C-term) in T47D cell line lysates (35ug/lane). TSPAN12 (arrow) was detected using the purified Pab. 0.4 mL 6 to 8 Days
$484.00
Details
Human Rabbit Un-conjugated IF (p), IHC (p), WB Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-TSPAN12 Polyclonal Antibody, Unconjugated (ABIN2179830) at 1:200 followed by conjugation to the secondary antibody and DAB staining 100 μL 14 to 21 Days
$317.90
Details
Dog Rabbit Un-conjugated ELISA, WB 100 μL 2 to 3 Days
$446.88
Details
Human Rabbit Un-conjugated FACS, IHC, WB Western blot analysis of extracts of T47D cells, using TSPAN12 antibody (ABIN5972606). 100 μL 11 to 16 Days
$426.40
Details
Human Rabbit Un-conjugated WB Tetraspanin 12 antibody used at 0.25 ug/ml to detect target protein. 50 μg 9 to 11 Days
$449.29
Details

More Antibodies against TSPAN12 Interaction Partners

Human Tetraspanin 12 (TSPAN12) interaction partners

  1. Our data indicate FEVR status is associated with a significantly smaller FAZ, decreased vascular density in the fovea. In addition, patients with the LRP5 mutation had a milder phenotype than those with the FDZ4 or TSPAN12 mutations.

  2. This is the first study to report a group of patients with digenic familial exudative vitreoretinopathy (FEVR). In most affected eyes, the stage was more severe than stage 3. We speculate that the phenotype of FEVR is more severe in patients with digenic rather than monogenic variants of FEVR-related genes.

  3. We identified two novel heterozygous deletion mutations [LRP5, c.4053 DelC (p.Ile1351IlefsX88); TSPAN12, EX8Del] using targeted NGS as a causative mutation for Familial exudative vitreoretinopathy (FEVR).

  4. The screening of candidate genes namely NDP, FZD4 and TSPAN12 led to the identification of six major coding region variants in 36 ROP probands.

  5. The novel variant p.Cys189Arg in TSPAN12 was not identified in the affected 14-year-old daughter. Thus, we conclude that the heterozygous FZD4 missense variant c.349T>C most likely represents a causative dominant mutation in this family with FEVR.

  6. Probands with LRP5 or NDP mutations were mainly categorized into group III and IV, TSPAN12 mutations were mainly observed in probands with group IV and V FEVR.

  7. Among the detected mutations, LRP5 accounted for the largest proportion with a mean mutation rate of 16.1% (5/31, 16.1%), followed by NDP (3/31, 9.7%), FZD4 (2/31, 6.5%), TSPAN12 (1/31, 3.2%), and KIF11 (1/31, 3.2%). All the novel changes were predicted to be pathogenic by a series of bioinformatics analyses.

  8. FEVR-associated genes contributing to the disorder's autosomal dominant inheritance pattern in Korea, we determined that patients with TSPAN12 large deletions were more common than patients with single nucleotide variants in TSPAN12.

  9. TSPAN12 promotes chemoresistance and proliferation of small cell lung carcinoma under the regulation of miR-495.

  10. Several novel mutations (missense, non-stop and insertion) were detected in the coding regions of FZD4, TSPAN12 and ZNF408 genes among the unrelated vitreoretinopathy probands.The mutations in FZD4 and TSPAN12 were involved in autosomal dominant and autosomal recessive families and further validates the involvement of these gene in familial exudative vitreoretinopathy development.

  11. The authors report a case of familial exudative vitreoretinopathy in the spectrum of osteoporosis pseudoglioma syndrome associated with novel mutations of the LRP5 and TSPAN12 genes that resulted in a phenotype similar to bilateral persistent fetal vasculature.

  12. Among the patients with pathogenic mutations detected, FZD4 mutations accounted for the largest proportion of autosomal inheritance FEVR cases (13/18 patients, 72.2%), followed by LRP5 (4/18 patients, 22.2%) and TSPAN12 (1/18 patients, 5.6%).

  13. Here we describe a case of a female infant affected by cystic fibrosis and by a severe form of exudative vitreoretinopathy. In particular, we have detected the homozygous missense mutation c.668 T > C in TSPAN12.

  14. Novel mutation in TSPAN12 leads to autosomal recessive inheritance of congenital vitreoretinal disease with intra-familial phenotypic variability.

  15. Novel mutations have been described in the TSPAN12 gene in Chinese patients with familial exudative vitreoretinopathy.

  16. These results suggest that stroma-derived p53 plays a pivotal role in epithelial cancer progression and that TSPAN12 and CXCL6 are potential targets for lung cancer therapy.

  17. TSPAN12 plays a role in supporting primary tumor growth and suppressing metastasis.

  18. This study is the first report of recessive mutations in TSPAN12 and shows that patients with two mutant alleles have a severe form of FEVR or retinal dysplasia, whereas heterozygous family members have mild familial exudative vitreoretinopathy phenotypes

  19. The largest miRNA-146a-TSPAN12 response to stress of amyloidbeta peptide + tumor necrosis factoralpha is found in human neuronal glial cells from Alzheimer brain.

  20. We speculate that haploinsufficiency of TSPAN12 contributes to PHPV.

Mouse (Murine) Tetraspanin 12 (TSPAN12) interaction partners

  1. TSPAN12 promotes chemoresistance and proliferation of small cell lung carcinoma under the regulation of miR-495.

  2. Data indicate that Norrin multimers and TSPAN12 cooperatively promote multimerization of FZD4 and its associated proteins to elicit physiological levels of signaling.

TSPAN12 Antigen Profile

Protein Summary

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility.

Gene names and symbols associated with TSPAN12

  • tetraspanin-7 (LOC412465) antibody
  • tetraspanin 12 (TSPAN12) antibody
  • tetraspanin 12 (tspan12) antibody
  • tetraspanin 12 (Tspan12) antibody
  • 9030619E17 antibody
  • AI426782 antibody
  • AI663988 antibody
  • AW111457 antibody
  • EVR5 antibody
  • NET-2 antibody
  • NET2 antibody
  • Tm4sf12 antibody
  • zgc:63880 antibody

Protein level used designations for TSPAN12

tetraspanin 12 , tetraspanin-12 , transmembrane 4 superfamily member 12 , tspan-12 , tetraspan NET-2

GENE ID SPECIES
412465 Apis mellifera
100220256 Taeniopygia guttata
100603513 Nomascus leucogenys
394127 Danio rerio
417763 Gallus gallus
23554 Homo sapiens
612262 Canis lupus familiaris
613639 Bos taurus
269831 Mus musculus
362326 Rattus norvegicus
100174568 Pongo abelii
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