Anti-His Affinity Resin

Catalog No. ABIN2017624

Quick Overview for Anti-His Affinity Resin (ABIN2017624)

Target: His Tag

Application: Immunoprecipitation (IP), Purification (Purif), Separation (Sep)

Product Details

Reactivity: Tag

Purpose: The product is designed for purification and immunoprecipitation of His-tagged protein, especially for the sample failed by Ni-NTA Agarose Chromatography purification.

Specificity: Repeat usage: Generally the resin can be recycled at least 4 times. If maintained properly, the resin may be recycled 10 times with minor binding capability loss
Elution methods: Acid, alkaline and peptide competitive elutions
Reagents compatibility: Compatible with mostly used reagents at certain concentrations
Consistency: Lot-to-lot consistency proved by strict production procedure

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 50% resin in TBS with 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freezing the resin

Storage: 4 °C

Storage Comment: Stable at 2-8 °C for 1 year

Expiry Date: 12 months

References

Shi, Zhang, Jia, Zhao, Yu, Wang: "Identification and molecular characterization of a Spätzle-like protein from Chinese shrimp (Fenneropenaeus chinensis)." in: Fish & shellfish immunology, Vol. 27, Issue 5, pp. 610-7, (2009) (PubMed).

Target Details

Target: His Tag

Target Type: Tag