CUT&RUN Negative Control

Catalog No. ABIN6923140

Quick Overview for CUT&RUN Negative Control (ABIN6923140)

Application: Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag)

Product Details

Purpose: CUT&RUN Negative Control antibody of our CUT&RUN Sets.

Specificity: Anti-Rabbit IgG (H&L) generated in guinea pig detects rabbit Immunoglobulin G.

Cross-Reactivity (Details): No reaction was observed against Goat, Human and Mouse Serum Proteins.

Characteristics: Polyclonal Guinea Pig Anti-Rabbit IgG Antibody, Pre-Adsorbed

Purification: Immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities. 

Sterility: Sterile filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 μg/μL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below.

Expiry Date: 12 months

References

Cavalheiro, Girardot, Viales, Pollex, Cao, Lacour, Feng, Rabinowitz, Furlong: "CTCF, BEAF-32, and CP190 are not required for the establishment of TADs in early Drosophila embryos but have locus-specific roles." in: Science advances, Vol. 9, Issue 5, pp. eade1085, (2023) (PubMed).

Manceau, Richard Albert, Lollini, Greenberg, Gilardi-Hebenstreit, Ribes: "Divergent transcriptional and transforming properties of PAX3-FOXO1 and PAX7-FOXO1 paralogs." in: PLoS genetics, Vol. 18, Issue 5, pp. e1009782, (2022) (PubMed).