ERBB3 antibody (C-Term)

Catalog No. ABIN100396

Supplier Product No.: 200-401-205

Product Details anti-ERBB3 Antibody

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERBB3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 200-401-205

Supplier: Rockland

Purpose: HER3 Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Characteristics: Synonyms: rabbit anti-HER3 Antibody, HER, c erbB 3 antibody, c erbB3 antibody, ERBB3 antibody, ERBB3 protein antibody, erbB3 S antibody, Glial growth factor receptor antibody

Purification: Rabbit Anti-HER3 is an IgG fraction antibody purified from monospecific antiserum by Protein A chromatography followed by cross adsorption against GST and extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-HER3 whole rabbit serum was prepared by repeated immunizations with a HER3 fusion protein corresponding to amino acids 1283 to 1323 (40 amino acids) at the carboxy-terminus of human HER3.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-HER3 antibody has been tested by ELISA, immunoprecipitation, and western blot and is specifically designed for immunoprecipitation and can be used for immunoblotting in conjunction with a detection antibody like anti-phosphotyrosine.  Reactivity in other assays is likely, but has not been determined.  Recognition of HER3 is independent of the phosphorylation status of the molecule.  Human prostate cell lines (LNCap) or human breast cell line (MDA-453) are typically used as positive control sources. For immunoprecipitation use approximately 5 μL of the antibody. The immunoprecipitation mix contained the antibody, 25 μL of Protein A-agarose beads and 1.0 mL of lysate (lysate contains approximately 1.0 mg of total protein). This mixture is rotated overnight at 4 °C and then washed 3 times with lysis buffer (used to prepare the lysate). The resulting bead complex is dissolved in 20-30 μL of 3X SDS-PAGE sample buffer and approximately 15 μL is loaded per lane on an 8 % polyacrylamide gel. The combination of immunoprecipitation and immunoblotting was performing using the anti-HER3 antibody for immunoprecipitation followed by immunoblot detection using an anti-phosphotyrosine antibody. The complex is then reacted with HRP Goat-anti-Rabbit IgG and ECL for detection and shows a single HER3 band at 180 kDa. Researchers should determine optimal titers for other applications.

Western Blot Dilution: 1:500- 1:2,000

Immunoprecipitation Dilution: Use 5 μL antibody.

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-HER3 at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ERBB3

Target: ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

Alternative Name: ERBB3 (ERBB3 Products)

Background: Background: HER3 antibody is ideal for western blotting, ELISA and IP. The human Epidermal Growth Factor Receptor (EGFR) family is a family of receptor tyrosine kinases consisting of 4 members: EGFR, HER2, HER3 and HER4.  These receptors are expressed on a wide variety of tissue types and have been implicated in many human cancers, including breast, gastric, colon and prostate.  Strong expression of both HER3 and HER2 (also known as p185neu) by tumor cells can be associated with poor outcome for patients.  The ligand for HER3 and HER4 is called Neu Differentiation Factor (NDF) or Heregulin.  In some instances, antibodies against these receptors prove effective in combination therapy regimens for treatment of the disease.  Signaling through the EGFR family involves the combinatorial dimerization of the four known family members and is mediated by at least 8 different hormones, including EGF and the heregulins.  Heregulin binding and tyrosine phosphorylation by HER3 or HER4 is enhanced in the presence of HER2.  Structurally the members of the EGFR family are 1210 to 1342 amino acids in length with approximately 650 amino acids of the extracellular domain being heavily glycosylated.  The extracellular domains show up to 70 % sequence homology.

Gene ID: 2065

NCBI Accession: NP_001005915

UniProt: P21860

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway