ERBB3 antibody (V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian)) (C-Term)

Details for Product anti-ERBB3 Antibody No. ABIN100396
Antigen
Epitope
C-Term, AA 128-3
46
38
29
27
20
19
17
15
15
15
15
15
14
12
8
7
7
6
6
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
466
248
222
10
5
3
3
3
3
3
2
2
1
1
1
Host
Rabbit
359
124
10
1
Clonality
Polyclonal
Conjugate
This ERBB3 antibody is un-conjugated
21
14
14
14
14
14
14
10
8
6
5
5
5
5
5
5
3
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
282
196
148
75
71
70
67
42
12
10
7
7
5
4
3
2
1
Options
Immunogen Anti-HER3 whole rabbit serum was prepared by repeated immunizations with a HER3 fusion protein corresponding to amino acids 1283 to 1323 (40 amino acids) at the carboxy-terminus of human HER3.
Immunogentype:Recombinant
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Sterility Sterile filtered
Antigen
Alternative Name HER3 (ERBB3 Antibody Abstract)
Background The human Epidermal Growth Factor Receptor (EGFR) family is a family of receptor tyrosine kinases consisting of 4 members: EGFR, HER2, HER3 and HER4.  These receptors are expressed on a wide variety of tissue types and have been implicated in many human cancers, including breast, gastric, colon and prostate.  Strong expression of both HER3 and HER2 (also known as p185neu) by tumor cells can be associated with poor outcome for patients.  The ligand for HER3 and HER4 is called Neu Differentiation Factor (NDF) or Heregulin.  In some instances, antibodies against these receptors prove effective in combination therapy regimens for treatment of the disease.  Signaling through the EGFR family involves the combinatorial dimerization of the four known family members and is mediated by at least 8 different hormones, including EGF and the heregulins.  Heregulin binding and tyrosine phosphorylation by HER3 or HER4 is enhanced in the presence of HER2.  Structurally the members of the EGFR family are 1210 to 1342 amino acids in length with approximately 650 amino acids of the extracellular domain being heavily glycosylated.  The extracellular domains show up to 70% sequence homology.
Synonyms: c erbB 3 antibody, c erbB3 antibody, ERBB3 antibody, ERBB3 protein antibody, erbB3 S antibody, Glial growth factor receptor antibody
Gene ID 2065
UniProt P21860
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Anti-HER3 antibody is specifically designed for immunoprecipitation and can be used for immunoblotting in conjunction with a detection antibody like anti-phosphotyrosine.  Reactivity in other assays is likely, but has not been determined.  Recognition of HER3 is independent of the phosphorylation status of the molecule.  Human prostate cell lines (LNCap) or human breast cell line (MDA-453) are typically used as positive control sources. For immunoprecipitation use approximately 5 µL of the antibody. The immunoprecipitation mix contained the antibody, 25 µL of Protein A-agarose beads and 1.0 ml of lysate (lysate contains approximately 1.0 mg of total protein). This mixture is rotated overnight at 4°C and then washed 3 times with lysis buffer (used to prepare the lysate). The resulting bead complex is dissolved in 20-30 µL of 3X SDS-PAGE sample buffer and approximately 15 µL is loaded per lane on an 8% polyacrylamide gel. The combination of immunoprecipitation and immunoblotting was performing using the anti-HER3 antibody for immunoprecipitation followed by immunoblot detection using an anti-phosphotyrosine antibody. The complex is then reacted with HRP Goat-anti-Rabbit IgG and ECL for detection and shows a single HER3 band at 180kDa. Researchers should determine optimal titers for other applications.
Restrictions For Research Use only
Format Liquid
Concentration 2.5 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Image no. 1 for anti-V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3) (AA 128-3), (C-Term) antibody (ABIN100396) Combined immunoprecipitation and western blot of a human cell lysate using anti-HER3 ...
Image no. 2 for anti-V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3) (AA 128-3), (C-Term) antibody (ABIN100396) anti-V-Erb-B2 Erythroblastic Leukemia Viral Oncogene Homolog 3 (Avian) (ERBB3) (AA 128-3), (C-Term) antibody (Image 2)