Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (pThr501) antibody

Details for Product No. ABIN100676
Antigen
Epitope
pThr501
15
13
11
9
9
9
7
7
6
6
6
5
3
3
3
2
1
1
1
1
Reactivity
Human
126
62
30
2
2
2
2
1
1
1
1
1
Host
Rabbit
123
22
Clonality
Polyclonal
Conjugate
Un-conjugated
8
5
5
4
4
4
4
4
4
4
4
4
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
110
60
45
20
18
7
3
3
2
2
1
Options
Immunogen IKKe phospho peptide corresponding to a region of the human protein surrounding pT501 conjugated to KLH.
Isotype IgG
Characteristics Concentration Definition: by UV absorbance at 280 nm
Sterility Sterile filtered
Antigen
Alternative Name IKK E (IKBKE Antibody Abstract)
Background Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity.  IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg.   Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKepsilon is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFα or IL-1.  LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa. Anti-IKKε pT501 antibody is ideal for investigators involved in NFkappaB and apoptosis reasearch.
Synonyms: I kappa B kinase epsilon antibody, IkBKE antibody, IKBKE protein antibody, Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody, Inhibitor of nuclear factor kappa B kinase subunit epsilon antibody, IKKE, IKKI, KIAA0151
Gene ID 9641
UniProt Q9R0T8, Q14164
Pathways TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
Application Notes IKKε pT501 antibody is suitable for use in ELISA, western blotting, and although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. An 85 kDa band corresponding to human IKKe is detected.  HeLa cells or TNF inducible KBM-5 cells can be used as a positive control. Researchers should determine optimal titers for other applications.
Restrictions For Research Use only
Format Liquid
Concentration 1.15 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Sweeney, Mo, Firestein: "Antiviral gene expression in rheumatoid arthritis: role of IKKepsilon and interferon regulatory factor 3." in: Arthritis and rheumatism, Vol. 56, Issue 3, pp. 743-52, 2007 (PubMed).