IKKi/IKKe antibody (pThr501)

Catalog No. ABIN100676

Product Details anti-IKKi/IKKe Antibody

Target: IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))

Binding Specificity: pThr501

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IKKi/IKKe antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Sterility: Sterile filtered

Immunogen: IKKe phospho peptide corresponding to a region of the human protein surrounding pT501 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes: IKKε pT501 antibody is suitable for use in ELISA, western blotting, and although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. An 85 kDa band corresponding to human IKKe is detected.  HeLa cells or TNF inducible KBM-5 cells can be used as a positive control. Researchers should determine optimal titers for other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.15 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-IKKi/IKKe antibody (ABIN100676)

DeDiego, Nogales, Martinez-Sobrido, Topham: "Interferon-Induced Protein 44 Interacts with Cellular FK506-Binding Protein 5, Negatively Regulates Host Antiviral Responses, and Supports Virus Replication." in: mBio, Vol. 10, Issue 4, (2020) (PubMed).

Sweeney, Mo, Firestein: "Antiviral gene expression in rheumatoid arthritis: role of IKKepsilon and interferon regulatory factor 3." in: Arthritis and rheumatism, Vol. 56, Issue 3, pp. 743-52, (2007) (PubMed).

Target Details for IKKi/IKKe

Target: IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))

Alternative Name: IKK E (IKBKE Products)

Synonyms: zgc:100844 antibody, IKBKE antibody, MGC130907 antibody, IKK-E antibody, IKK-i antibody, IKKE antibody, IKKI antibody, AW558201 antibody, IKKepsilon antibody, Ikki antibody, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon antibody, inhibitor of nuclear factor kappa B kinase subunit epsilon antibody, inhibitor of nuclear factor kappa B kinase subunit epsilon L homeolog antibody, inhibitor of kappaB kinase epsilon antibody, IKBKE antibody, ikbke antibody, ikbke.L antibody, Ikbke antibody

Background: Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity.  IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg.   Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKepsilon is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFα or IL-1.  LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa. Anti-IKKε pT501 antibody is ideal for investigators involved in NFkappaB and apoptosis reasearch.
Synonyms: I kappa B kinase epsilon antibody, IkBKE antibody, IKBKE protein antibody, Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon antibody, Inhibitor of nuclear factor kappa B kinase subunit epsilon antibody, IKKE, IKKI, KIAA0151

Gene ID: 9641

UniProt: Q9R0T8, Q14164

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades