IKKi/IKKe antibody (pThr501)
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- Target See all IKKi/IKKe (IKBKE) Antibodies
- IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))
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Binding Specificity
- pThr501
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This IKKi/IKKe antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Supplier Product No.
- 600-401-267
- Supplier
- Rockland
- Purpose
- IKK E phospho T501 Antibody
- Cross-Reactivity (Details)
- This phospho specific polyclonal antibody is specific for phosphorylated pT501 human IKKe.
- Characteristics
- Synonyms: rabbit anti-IKK epsilon pT501 antibody, rabbit anti-IKKE pT501 antibody, I kappa B kinase epsilon antibody, IKK-epsilon, IKK-E, Inducible I kappa-B kinase, IkBKE antibody, IKBKE protein antibody, Inhibitor of nuclear factor kappa B kinase subunit epsilon antibody, IKKE, IKKI
- Purification
- Anti-IKKε pT501 antibody was affinity purified from monospecific antiserum by immunoaffinity purification against the phosphopeptide and cross adsorption against the non-phosphorylated form of the peptide followed by non-adsorption against a non-specific peptide backbone to further reduce non-specific reactivity.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: IKKe phospho peptide corresponding to a region of the human protein surrounding pT501 conjugated to KLH.
Immunogen Type: Conjugated Peptide
- Isotype
- IgG
- Top Product
- Discover our top product IKBKE Primary Antibody
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- Application Notes
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Application Note: IKKε pT501 antibody is tested in ELISA, western blotting, and although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. An 85 kDa band corresponding to human IKKe is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control. Researchers should determine optimal titers for other applications.
Western Blot Dilution: 1:500 - 1:3,000
ELISA Dilution: 1:5,000 - 1:25,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.15 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.1 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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Interferon-Induced Protein 44 Interacts with Cellular FK506-Binding Protein 5, Negatively Regulates Host Antiviral Responses, and Supports Virus Replication." in: mBio, Vol. 10, Issue 4, (2020) (PubMed).
: "Antiviral gene expression in rheumatoid arthritis: role of IKKepsilon and interferon regulatory factor 3." in: Arthritis and rheumatism, Vol. 56, Issue 3, pp. 743-52, (2007) (PubMed).
: "
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Interferon-Induced Protein 44 Interacts with Cellular FK506-Binding Protein 5, Negatively Regulates Host Antiviral Responses, and Supports Virus Replication." in: mBio, Vol. 10, Issue 4, (2020) (PubMed).
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- Target
- IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))
- Alternative Name
- IKBKE (IKBKE Products)
- Background
- Background: Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity. IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKepsilon is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFα or IL-1. LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa. Anti-IKKε pT501 antibody is ideal for investigators involved in NFkappaB and apoptosis research.
- Gene ID
- 9641
- UniProt
- Q14164
- Pathways
- TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
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