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IKKi/IKKe antibody (pThr501)

IKBKE Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated
Rockland
Catalog No. ABIN100676
Supplier Product No.: 600-401-267
  • Target See all IKKi/IKKe (IKBKE) Antibodies
    IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))
    Binding Specificity
    • 15
    • 9
    • 8
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pThr501
    Reactivity
    • 57
    • 13
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 61
    • 7
    Rabbit
    Clonality
    • 53
    • 15
    Polyclonal
    Conjugate
    • 31
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This IKKi/IKKe antibody is un-conjugated
    Application
    • 52
    • 27
    • 16
    • 16
    • 13
    • 13
    • 5
    • 4
    • 4
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Supplier Product No.
    600-401-267
    Supplier
    Rockland
    Purpose
    IKK E phospho T501 Antibody
    Cross-Reactivity (Details)
    This phospho specific polyclonal antibody is specific for phosphorylated pT501 human IKKe.
    Characteristics
    Synonyms: rabbit anti-IKK epsilon pT501 antibody, rabbit anti-IKKE pT501 antibody, I kappa B kinase epsilon antibody, IKK-epsilon, IKK-E, Inducible I kappa-B kinase, IkBKE antibody, IKBKE protein antibody, Inhibitor of nuclear factor kappa B kinase subunit epsilon antibody, IKKE, IKKI
    Purification
    Anti-IKKε pT501 antibody was affinity purified from monospecific antiserum by immunoaffinity purification against the phosphopeptide and cross adsorption against the non-phosphorylated form of the peptide followed by non-adsorption against a non-specific peptide backbone to further reduce non-specific reactivity.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: IKKe phospho peptide corresponding to a region of the human protein surrounding pT501 conjugated to KLH.

    Immunogen Type: Conjugated Peptide

    Isotype
    IgG
    Top Product
    Discover our top product IKBKE Primary Antibody
  • Application Notes

    Application Note: IKKε pT501 antibody is tested in ELISA, western blotting, and although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. An 85 kDa band corresponding to human IKKe is detected.  HeLa cells or TNF inducible KBM-5 cells can be used as a positive control. Researchers should determine optimal titers for other applications.

    Western Blot Dilution: 1:500 - 1:3,000

    ELISA Dilution: 1:5,000 - 1:25,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.15 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.1 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • DeDiego, Nogales, Martinez-Sobrido, Topham: "Interferon-Induced Protein 44 Interacts with Cellular FK506-Binding Protein 5, Negatively Regulates Host Antiviral Responses, and Supports Virus Replication." in: mBio, Vol. 10, Issue 4, (2020) (PubMed).

    Sweeney, Mo, Firestein: "Antiviral gene expression in rheumatoid arthritis: role of IKKepsilon and interferon regulatory factor 3." in: Arthritis and rheumatism, Vol. 56, Issue 3, pp. 743-52, (2007) (PubMed).

  • Target
    IKKi/IKKe (IKBKE) (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE))
    Alternative Name
    IKBKE (IKBKE Products)
    Background
    Background: Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity.  IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg.   Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKepsilon is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFα or IL-1.  LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa. Anti-IKKε pT501 antibody is ideal for investigators involved in NFkappaB and apoptosis research.
    Gene ID
    9641
    UniProt
    Q14164
    Pathways
    TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
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