SDCCAG8 antibody (Internal Region)

Catalog No. ABIN1019666

The Goat Polyclonal anti-SDCCAG8 antibody has been validated for WB, ELISA and IHC. It is suitable to detect SDCCAG8 in samples from Human.

Quick Overview for SDCCAG8 antibody (Internal Region) (ABIN1019666)

Target: SDCCAG8 (serologically Defined Colon Cancer Antigen 8 (SDCCAG8))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SDCCAG8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-SDCCAG8 Antibody

Binding Specificity: Internal Region

Purpose: SDCCAG8

Sequence: TINDQSQYIH HLEAE

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-TINDQSQYIHHLEAE, from the internal region of the protein sequence according to NP_006633.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5 μg/mL.

Western Blot: Approx 80 kDa band observed in lysates of cell line HEK293 (calculated MW of 82.7 kDa according to NP_006633.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SDCCAG8

Target: SDCCAG8 (serologically Defined Colon Cancer Antigen 8 (SDCCAG8))

Alternative Name: SDCCAG8

Background: SDCCAG8, serologically defined colon cancer antigen 8, BBS16, CCCAP, NPHP10, NY-CO-8, SLSN7, hCCCAP, OTTHUMP00000037913, antigen NY-CO-8, centrosomal colon cancer autoantigen protein

Gene ID: 10806, 76816, 305002

NCBI Accession: NP_006633

Pathways: M Phase, Tube Formation