KIT antibody (APC)

Catalog No. ABIN1027690

Product Details anti-KIT Antibody

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivity: Human, Cow, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This KIT antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Specificity: The mouse monoclonal antibody 104D2 detects extracellular part of CD117 / c-Kit protooncogen.
HLDA VI, WS Code C-30

Cross-Reactivity (Details): Human, Non-Human Primates, Bovine

Immunogen: MOLM-1 megakaryocytic cells

Clone: 104D2

Isotype: IgG1

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-KIT antibody (ABIN1027690)

Stevenson, McGlynn, Hodge, McLinden, George, Davies, Shiels: "Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations." in: Stem cells and development, Vol. 18, Issue 10, pp. 1389-98, (2009) (PubMed).

Nagano, Yamashita, Hamada, Ohneda, Kimura, Nakagawa, Shibuya, Yoshikawa, Ohneda: "Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood." in: Blood, Vol. 110, Issue 1, pp. 151-60, (2007) (PubMed).

Wihlidal, Varga, Pfeilstöcker, Karlic: "Expression and functional significance of osteocalcin splicing in disease progression of hematological malignancies." in: Leukemia research, Vol. 30, Issue 10, pp. 1241-8, (2006) (PubMed).

Blair, Sutherland: "Primitive acute myeloid leukemia cells with long-term proliferative ability in vitro and in vivo lack surface expression of c-kit (CD117)." in: Experimental hematology, Vol. 28, Issue 6, pp. 660-71, (2000) (PubMed).

Yoshino, Ami, Terao, Tashiro, Honda: "Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies." in: Experimental animals / Japanese Association for Laboratory Animal Science, Vol. 49, Issue 2, pp. 97-110, (2000) (PubMed).

Broudy, Lin, Liles, Corey, OLaughlin, Mou, Linnekin: "Signaling via Src family kinases is required for normal internalization of the receptor c-Kit." in: Blood, Vol. 94, Issue 6, pp. 1979-86, (1999) (PubMed).

Broudy, Lin, Bühring, Komatsu, Kavanagh: "Analysis of c-kit receptor dimerization by fluorescence resonance energy transfer." in: Blood, Vol. 91, Issue 3, pp. 898-906, (1998) (PubMed).

Rappold, Ziegler, Köhler, Marchetto, Rosnet, Birnbaum, Simmons, Zannettino, Hill, Neu, Knapp, Alitalo, Alitalo, Ullrich, Kanz, Bühring: "Functional and phenotypic characterization of cord blood and bone marrow subsets expressing FLT3 (CD135) receptor tyrosine kinase." in: Blood, Vol. 90, Issue 1, pp. 111-25, (1997) (PubMed).

Target Details for KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: CD117 (KIT Products)

Synonyms: C-Kit antibody, CD117 antibody, PBT antibody, SCFR antibody, Bs antibody, Fdc antibody, Gsfsco1 antibody, Gsfsco5 antibody, Gsfsow3 antibody, SCO1 antibody, SCO5 antibody, SOW3 antibody, Ssm antibody, Tr-kit antibody, W antibody, c-KIT antibody, c-kit antibody, MGF antibody, KIT antibody, C-KIT antibody, kit antibody, receptor antibody, sparse antibody, Xkl-1 antibody, kit-a antibody, kit-b antibody, kl1-A antibody, krk1 antibody, pbt antibody, scfr antibody, KIT proto-oncogene receptor tyrosine kinase antibody, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a antibody, KIT proto-oncogene receptor tyrosine kinase S homeolog antibody, KIT antibody, Kit antibody, kita antibody, kit antibody, kit.S antibody

Background: CD117 / c-Kit (stem cell factor receptor) is a 145 kDa receptor tyrosine kinase that regulates cell proliferation, adhesion, chemotaxis, apoptosis and other cell processes. Mutations of CD117 / c-Kit can lead to growth and progression of tumours. After binding of its ligand, SCF (stem cell factor), CD117 / c-Kit is autophosphorylated on its intracellular domains and activated. CD117 is expressed on pluripotent hematopoietic progenitor cells, mast cells and various cancer cells, e.g. acute myeloid leukemia cells.

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity