SLC17A7 antibody (AA 493-560)

Catalog No. ABIN1027710

This anti-SLC17A7 antibody is a Mouse Monoclonal antibody detecting SLC17A7 in WB, IHC and mIHC. Suitable for Rat. Independently validated for use in Multiplex Immunohistochemistry.

Quick Overview for SLC17A7 antibody (AA 493-560) (ABIN1027710)

Target: SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLC17A7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Multiplex Immunohistochemistry (mIHC)

Clone: S28-9

Product Details anti-SLC17A7 Antibody

Binding Specificity: AA 493-560

Specificity: Detects ~52 kDa. No cross-reactivity against VGlut2.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1

Isotype: IgG1

Application Details

Application Notes:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Comment:

1 μg/ml of ABIN1027710 was sufficient for detection of VGLut1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Independent Validation (mIHC)

Validation #104338 (Multiplex Immunohistochemistry)

by: Akoya Biosciences

No.: #104338

Date: 04/20/2021

Antigen: SLC17A7

Lot Number: 1205

Method validated: Multiplex Immunohistochemistry

Positive Control:

Fresh frozen mouse olfactory bulb

Negative Control:

Unlabeled control (mouse fresh frozen)

Notes:

Passed. The anti-SLC17A7 antibody ABIN1027710 labels areas of high glutamatergic afferent input throughout the murine olfactory bulb with a concentration in the internal plexiform and granule cell layer.

Validation Images

Murine fresh frozen coronal olfactory bulb section (Thickness = 5 µm) labeled with anti-SLC17A7 antibody ABIN1027710 (green; bound to fluorophore ATTO 550). Labeling is present throughout olfactory bulb layers with a concentration in the internal plexiform and granule cell layers. Map2 and PSD-95/DLG4 were labeled with ABIN125739 (blue; bound to fluorophore ATTO 550) and ABIN361694 (red; bound to fluorophore ATTO 550).

FFPE normal human cortex tissue section labeled with anti-SL17A7 antibody ABIN1027710 (green; bound to fluorophore ATTO 550) after EDTA antigen retrieval. MAP2 and dopamine transporter were labeledd with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 550) and anti-dopamine receptor antibody (blue; bound to fluorophore AF647).

Full Methods

Primary Antibody: ABIN1027710

Full Protocol:

• Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
• Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
• Conjugation of the anti-SLC17A7 antibody ABIN1027710 to an oligo barcode used to bind oligo-conjugated fluorophore ATTO 550.

Experimental Notes:

• No signal was detected in unlabeled specimens.

• Specific staining of Cortactin was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

• Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for SLC17A7

Target: SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

Alternative Name: VGLUT1

Background: VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).

Gene ID: 116638

NCBI Accession: NP_446311

UniProt: Q62634