DLD antibody

Catalog No. ABIN103577

Supplier Product No.: 200-4160-0100

This anti-DLD antibody is a Rabbit Polyclonal antibody detecting DLD in WB, ELISA and IP. Suitable for Pig.

Quick Overview for DLD antibody (ABIN103577)

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Reactivity: Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DLD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-DLD Antibody

Supplier Product No.: 200-4160-0100

Supplier: Rockland

Purpose: Lipoamide Dehydrogenase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lipoamide Dehydrogenase [Porcine Heart].

Characteristics: Synonyms: rabbit anti-Lipoamide Dehydrogenase Antibody, Dihydrolipoyl dehydrogenase mitochondrial, Dihydrolipoamide dehydrogenase, Lipoamide Dehydrogenase

Purification: Anti-Lipoamide Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Lipoamide Dehydrogenase [Porcine Heart]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Lipoamide Dehydrogenase has been tested by ELISA and western blot and is assayed against 1.0 μg of Lipoamide Dehydrogenase [Porcine Heart] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:7,000 to 1:30,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for DLD

Target: DLD (Dihydrolipoamide Dehydrogenase (DLD))

Alternative Name: DLD LAD

Background: Background: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as an E3 component of the alpha-ketoacid dehydrogenase complexes (pyruvate-, alpha-ketoglutarate-, and branched-chain amino acid-dehydrogenase complex). In monomeric form it has additional moonlighting function as serine protease. It is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

Gene ID: 397129, 118675

UniProt: P09623

Pathways: Ribonucleoside Biosynthetic Process, Cell RedoxHomeostasis