MIF antibody (AA 2-32)

Catalog No. ABIN103836

Supplier Product No.: 200-901-457

Product Details anti-MIF Antibody

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Binding Specificity: AA 2-32

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This MIF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 200-901-457

Supplier: Rockland

Purpose: MIF Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum.

Characteristics: Synonyms: chicken Anti-Macrophage migration inhibitory factor antibody, chicken Anti-MIF antibody, Phenylpyruvate tautomerase antibody, GIF antibody, GLIF antibody, Glycosylation inhibiting factor antibody, Macrophage migration inhibitory factor antibody, MIF protein antibody

Purification: This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to aa 2-32 of Human MIF conjugated to keyhole limpet hemocyanin (KLH). MIF is a proinflammatory cytokine that plays an important role in systemic inflammatory events.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This IgY fraction antibody of anti-Human macrophage migration inhibitory factor (MIF) has been tested for use in ELISA and immunoblotting.  Although not tested, this antibody may also be useful for neutralization assays, immunohistochemistry and flow cytometry.  The antibody recognizes 12,300 MW mature human MIF. The MIF gene encodes a protein of 115 aa.  The initiating methionine is cleaved leaving a mature protein of 114 aa. Reactivity in other immunoassays is unknown.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 5.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MIF

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Alternative Name: MIF (MIF Products)

Background: Background: Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine. It is involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. It counteracts the anti-inflammatory activity of glucocorticoids. MIF has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

Gene ID: 4282

UniProt: P14174

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling