Histone H4 antibody (meLys20)

Catalog No. ABIN7539896

This anti-Histone H4 antibody is a Mouse Monoclonal antibody detecting Histone H4 in WB, IF, ELISA, ChIP, DB, ICC, IHC (p) and FACS. Suitable for Human.

Quick Overview for Histone H4 antibody (meLys20) (ABIN7539896)

Target: Histone H4

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 5E10-D8

Product Details anti-Histone H4 Antibody

Binding Specificity: meLys20

Specificity: Specific for K20 mono-methylated histone H4, no cross-reactivity with K20 tri-, di-, or unmethylated histone H4, no cross-reactivity with histone H3.

Cross-Reactivity: Arabidopsis thaliana, Drosophila melanogaster, Mammalian, Xenopus laevis

Cross-Reactivity (Details): Human. Predicted to work with: Xenopus laevis, Arabidopsis thaliana, Fruit fly (Drosophila melanogaster), all Mammals

Purification: Protein G

Immunogen: Synthetic peptide within Human Histone H4 (mono methyl K20)(Immunogen sequence: 15-AKRHR(Kme)VLRDN-25 (branched))

Isotype: IgG1

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try, WB: Use a concentration of 1 μg/mL. Detects a band of approximately 13 kDa (predicted molecular weight: 11 kDa).Can be blocked with Histone H4 peptide - mono methyl K20 

Comment:

Myeloma, fusion partners: X63-Ag8.653

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody (from supernatant) containing PBS + 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Histone H4

Target: Histone H4

Background: Synonyms:
H4F2 antibody,H4FN antibody,HIST1H4 antibody,HIST2H4 antibody,HISTH4H4 antibody,methyl histone H4 antibody,methylated histone H4 antibody

Gene ID: 318846, 3771854, 3771893

OMIM: 142750

UniProt: P59259, P84040, P02304, P62805, P62799