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Histone H4 antibody (3meLys20)

This Mouse Monoclonal antibody specifically detects Histone H4 in WB, ELISA, ChIP and DB. It exhibits reactivity toward Human and C. elegans.
Catalog No. ABIN1042606

Quick Overview for Histone H4 antibody (3meLys20) (ABIN1042606)

Target

See all Histone H4 Antibodies
Histone H4

Reactivity

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Human, C. elegans

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This Histone H4 antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB)

Clone

6F8-D9
  • Binding Specificity

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    3meLys20

    Specificity

    Specific for K20 tri-methylated histone H4, weak cross-reactivity with K20 di-methylated histone H4, but not mono- or unmethylated K20, no cross-reactivity with histone H3

    Cross-Reactivity

    Arabidopsis thaliana, Drosophila melanogaster, Mammalian, Xenopus laevis

    Cross-Reactivity (Details)

    Human, Caenorhabditis elegans. Predicted to work with: Xenopus laevis, Arabidopsis thaliana, Fruit fly (Drosophila melanogaster), all Mammals

    Purification

    Protein G

    Immunogen

    Synthetic peptide of Human Histone H4 (Immunogen sequence: 15-AKRHR(Kme3)VLRDN-25 (branched))

    Isotype

    IgG1
  • Application Notes

    Optimal antibody dilution should be determined by titration, however as a guideline try, WB: Use at 1:4000. Predicted molecular weight: 11 kDa.

    Comment

    Myeloma, fusion partners: X63-Ag8.653

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Purified antibody (from supernatant) containing PBS + 0.1 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Target

    Histone H4

    Background

    Synonyms:
    H4F2 antibody,H4FN antibody,HIST1H4 antibody,HIST2H4 antibody,HISTH4H4 antibody,methyl histone H4 antibody,methylated histone H4 antibody

    Gene ID

    318846, 3771854, 3771893

    OMIM

    142750

    UniProt

    P59259, P84040, P02304, P62805, P62799
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