LGR4 antibody (Internal Region)

Catalog No. ABIN1043731

Supplier Product No.: 200-301-b46

This Mouse Monoclonal antibody specifically detects LGR4 in ELISA, WB and IHC. It exhibits reactivity toward Human, Chimpanzee and Macaqueand has been mentioned in 1 publication.

Quick Overview for LGR4 antibody (Internal Region) (ABIN1043731)

Target: LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))

Reactivity: Human, Chimpanzee, Macaque

Host: Mouse

Clonality: Monoclonal

Conjugate: This LGR4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 6G8-B3-G5-C3

Product Details anti-LGR4 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 200-301-b46

Supplier: Rockland

Purpose: LGR4 Antibody

Cross-Reactivity (Details): This antibody is specific for human LGR4 protein.

Characteristics: Synonyms: mouse anti-LGR4 antibody, mouse anti-LGR 4 antibody, leucine-rich repeat-containing G protein-coupled receptor 4

Purification: Anti-LGR4 Antibody product was purified from concentrated tissue culture supernate by Protein A chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This LGR4 antibody was produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human LGR4 protein. The hybridoma was produced by fusing BALB/c mouse splenocytes and mouse myeloma SP2/O cells using conventional technology.

Immunogen Type: Conjugated Peptide

Isotype: IgG2b kappa

Application Details

Application Notes:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: Anti-LGR4 antibody has been tested by ELISA and western blotting, and is suitable in immunohistochemistry. Expect a band approximately 102 kDa in size corresponding to LGR4 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:20,000 - 1:100,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-LGR4 Monoclonal Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge antibody if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-LGR4 antibody (ABIN1043731)

Weng, Luo, Cheng, Jin, Zhou, Qu, Tu, Ai, Li, Wang, Martin, Amendt, Liu: "Deletion of G protein-coupled receptor 48 leads to ocular anterior segment dysgenesis (ASD) through down-regulation of Pitx2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 16, pp. 6081-6, (2008) (PubMed).

Target Details for LGR4

Target: LGR4 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 4 (LGR4))

Alternative Name: LGR4

Background: Background: LGR4, also known as leucine-rich repeat-containing G protein-coupled receptor 4, is a G protein-coupled receptors (GPCRs). GPCRs are membrane bound proteins that play key roles in a variety of physiologic functions. Members of the leucine-rich GPCR (LGR) family, such as GPR48, have multiple N-terminal leucine-rich repeats (LRRs) and a 7-transmembrane domain. LGR4 is an orphan GPCR reported to be expressed in steroidogenic tissues such as placenta, ovary, testis, adrenal, pancreas, prostate, and thyroid, as well as in spinal cord, stomach, heart, and kidney.

Gene ID: 55366, 157694513

UniProt: Q8N537