TIMP4 antibody

Catalog No. ABIN1043743

Supplier Product No.: 200-301-e65

Product Details anti-TIMP4 Antibody

Target: TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIMP4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 200-301-e65

Supplier: Rockland

Purpose: TIMP4 Antibody

Cross-Reactivity (Details): TIMP4 antibody is specific for human TIMP4 protein.

Characteristics: Synonyms: mouse anti-TIMP4 antibody, Tissue inhibitor of metalloproteinase-4, Metalloproteinase inhibitor 4, TIMP-4, TIMP 4, TIMP4 antibody, anti-TIMP-4 antibody

Purification: Anti-TIMP4 was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-TIMP4 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with full length human TIMP4 protein followed by hybridoma development.

Immunogen Type: Recombinant Protein

Clone: 9:4-7

Isotype: IgG2b kappa

Application Details

Application Notes:

Immunohistochemistry Dilution: 5-10 μg/mL

Application Note: Anti-TIMP4 antibody has been tested for use in ELISA, Native - Western, and IHC (P). SDS-PAGE - Western Blot not useful for endogenous detection. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:2,000 to 1:10,000

ELISA Dilution: 1:20,000 to 1:100,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-TIMP4 antibody (ABIN1043743)

Donover, Wojciechowski, Thirumaran, Zemba-Palko, Prendergast, Wallon: "Development of a monoclonal antibody that specifically detects tissue inhibitor of metalloproteinase-4 (TIMP-4) in formalin-fixed, paraffin-embedded human tissues." in: Journal of cellular biochemistry, Vol. 110, Issue 5, pp. 1255-61, (2010) (PubMed).

Target Details for TIMP4

Target: TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

Alternative Name: TIMP4 (TIMP4 Products)

Background: Background: Tissue inhibitor of metalloproteinase-4 (TIMP-4) is member of the TIMP family of inhibitors of matrix metalloproteinase (MMP) activities. In addition, TIMPs have non-MMP associated functions, including roles in growth promotion, apoptosis, and angiogenesis. All four members of the TIMP gene family form a similar secondary structure due to the formation of six highly conserved disulfide bonds. It has been reported that some TIMPs, TIMP4 included, can bind a tetraspanin in the plasma membrane, initiating an intra-cellular signaling cascade. Elevated levels of TIMP4 have been associated with breast cancer progression and heart disease.

Gene ID: 7079

NCBI Accession: NM_003256

UniProt: Q99727