beta Actin antibody (N-Term)

Catalog No. ABIN1043747

Supplier Product No.: 200-301-e69

The Mouse Monoclonal anti-beta Actin antibody has been validated for WB, ELISA and IHC. It is suitable to detect beta Actin in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for beta Actin antibody (N-Term) (ABIN1043747)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 3H2-E4-E12

Product Details anti-beta Actin Antibody

Binding Specificity: N-Term

Supplier Product No.: 200-301-e69

Supplier: Rockland

Purpose: Beta Actin Antibody

Cross-Reactivity (Details): beta Actin antibody is specific for human and mouse ß Actin protein.

Characteristics: Synonyms: mouse anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

Purification: Anti-beta Actin was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with ß-Actin peptide near the N-Terminus followed by hybridoma development.

Immunogen Type: Conjugated Peptide

Isotype: IgG2a lambda

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-beta Actin antibody has been tested for use in ELISA, immunohistochemistry, and Western Blot. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:5000-1:50000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-Beta Actin at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-beta Actin antibody (ABIN1043747)

Carvajal, Vallejos, Walters, Contreras, Hertz, Olivares, Cáceres, Pino, Letelier, Thompson, López-Lastra: "Structural domains within the HIV-1 mRNA and the ribosomal protein S25 influence cap-independent translation initiation." in: The FEBS journal, Vol. 283, Issue 13, pp. 2508-27, (2017) (PubMed).

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: ACTB

Background: Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.

Gene ID: 11461

NCBI Accession: NP_001092

UniProt: P60709

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction