Interferon gamma antibody
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- Target See all Interferon gamma (IFNG) Antibodies
- Interferon gamma (IFNG)
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Reactivity
- Cow
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Interferon gamma antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Supplier Product No.
- 201-401-c41
- Supplier
- Rockland
- Purpose
- Interferon Gamma Antibody
- Cross-Reactivity (Details)
- This antibody is specific for bovine IFN gamma protein.
- Characteristics
- Synonyms: rabbit anti-IFN gamma, rabbit anti-Interferon gamma, IFNG, BoIFNG
- Purification
- This product was protein A purified from monospecific antiserum by chromatography.
- Immunogen
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Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to the 143 amino acids of the mature Bovine IFN Gamma protein.
Immunogen Type: Recombinant Protein
- Isotype
- IgG
- Top Product
- Discover our top product IFNG Primary Antibody
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- Application Notes
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Application Note: This protein A purified IFN-gamma antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 16.9 kDa in size corresponding to bovine IFN gamma by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:500-1:2,000
ELISA Dilution: 1:20,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.59 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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A dendritic cell-targeted chimeric hepatitis B virus immunotherapeutic vaccine induces both cellular and humoral immune responses in vivo." in: Human vaccines & immunotherapeutics, Vol. 16, Issue 4, pp. 779-792, (2020) (PubMed).
: "Interferon-gamma: an overview of signals, mechanisms and functions." in: Journal of leukocyte biology, Vol. 75, Issue 2, pp. 163-89, (2004) (PubMed).
: "
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A dendritic cell-targeted chimeric hepatitis B virus immunotherapeutic vaccine induces both cellular and humoral immune responses in vivo." in: Human vaccines & immunotherapeutics, Vol. 16, Issue 4, pp. 779-792, (2020) (PubMed).
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- Target
- Interferon gamma (IFNG)
- Alternative Name
- IFN gamma (IFNG Products)
- Background
- Background: Interferon-gamma (IFN-gamma) is a dimerized soluble cytokine that is the only member of the type II class interferon. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-gamma belongs. IFN-gamma, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN-gamma expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN-gamma in the immune system stems in part from its ability to inhibit viral replication directly, but, most important, derives from its immunostimulatory and immunomodulatory effects. IFN-gamma is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.
- Gene ID
- 281237
- NCBI Accession
- NP_776511
- UniProt
- A9QXB7
- Pathways
- Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy
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