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Serotonin Receptor 6 antibody (HTR6) (C-Term) Primary Antibody

HTR6 Reactivity: Human IHC, IHC (p) Host: Rabbit Polyclonal
Catalog No. ABIN1048967
$484.00
Plus shipping costs $45.00
50 μg
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Delivery in 11 to 14 Business Days
  • Target
    Binding Specificity
    C-Term
    Reactivity
    Human
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    This Serotonin Receptor 6 antibody is un-conjugated
    Application
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human HTR6 / 5-HT6. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla (100%) Monkey, Horse (94%) Gibbon, Dog, Rabbit (88%) Elephant (82%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 17 amino acid peptide from C-terminus of human HTR6 / 5-HT6. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Monkey, Horse (94%), Gibbon, Dog, Rabbit (88%), Elephant (82%).

    Type of Immunogen: Synthetic peptide
  • Target
    Alternative Name
    HTR6 / 5-HT6 Receptor (HTR6 Antibody Abstract)
    Background
    Name/Gene ID: HTR6
    Subfamily: Serotonin
    Family: GPCR

    Synonyms: HTR6, 5-HT6, 5-HT6R, 5-HT-6, 5-hydroxytryptamine receptor 6, 5-HT6 receptor, 5-HT6 serotonin receptor, 5-hydroxytryptamine 6 receptor, Serotonin 6 receptor, Serotonin receptor 6, Serotonin 5-HT-6 receptor, Serotonin type 6 receptor
    Gene ID
    3362
    UniProt
    P50406
    Pathways
    JAK-STAT Signaling
  • Application Notes
    Approved: IHC, IHC-P (2.5 - 5 μg/mL)
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
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