Latrophilin 1 antibody (C-Term)

Catalog No. ABIN1049023

This Rabbit Polyclonal antibody specifically detects Latrophilin 1 in IHC and IHC (p). It exhibits reactivity toward Rat, Human, Mouse, Monkey and Hamster.

Quick Overview for Latrophilin 1 antibody (C-Term) (ABIN1049023)

Target: Latrophilin 1 (LPHN1)

Reactivity: Rat, Human, Mouse, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Latrophilin 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Latrophilin 1 Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: Human LPHN1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except NOL6 (50 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Platypus (100%) Bat, Bovine, Horse, Pig, Opossum (94%) Dog, Panda (88%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from C-terminus of human LPHN1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Platypus (100%), Bat, Bovine, Horse, Pig, Opossum (94%), Dog, Panda (88%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (1 - 2 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Latrophilin 1

Target: Latrophilin 1 (LPHN1)

Alternative Name: ADGRL1 / LPHN1

Background: Name/Gene ID: ADGRL1
Subfamily: Orphan-B
Family: GPCR

Synonyms: ADGRL1, Alpha-latrotoxin receptor, CIRL-1, CIRL1, Cl1 latrotoxin receptor, Latrophilin 1, Latrophilin-1, Lphh2, LPHN1, KIAA0821, LEC2, Cirl, CL1, Lectomedin-2

Gene ID: 22859

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis