TUBB3 antibody (Internal Region)

Catalog No. ABIN1049508

This anti-TUBB3 antibody is a Goat Polyclonal antibody detecting TUBB3 in WB and ELISA. Suitable for Human.

Quick Overview for TUBB3 antibody (Internal Region) (ABIN1049508)

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This TUBB3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-TUBB3 Antibody

Binding Specificity: Internal Region

Purpose: TUBB3

Sequence: DPSGNYVGDS D

Specificity: This antibody is expected to recognize isoform 1 (NP_006077.2) only.

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-DPSGNYVGDSD, from the internal region (near N terminus) of the protein sequence according to NP_006077.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 52 kDa band observed in lysates of cell line HepG2 (calculated MW of 50.4 kDa according to NP_006077.2). Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for TUBB3

Target: TUBB3 (Tubulin, beta 3 (TUBB3))

Alternative Name: TUBB3

Background: TUBB3, tubulin, beta 3, CDCBM, CFEOM3A, TUBB4, beta-4, OTTHUMP00000176846, class III beta-tubulin, tubulin beta-3 chain, tubulin beta-4 chain, tubulin beta-III

Gene ID: 10381, 22152, 246118

NCBI Accession: NP_006077

Pathways: Microtubule Dynamics, M Phase