Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

P21-Activated Kinases 1/2/3 (PAK1/2/3) (pThr423) antibody Primary Antibody

PAK1/2/3 Reactivity: Human IHC, ELISA, WB Host: Rabbit Polyclonal
Catalog No. ABIN105217
$613.50
Plus shipping costs $45.00 and $22.00 dry ice
100 μg
local_shipping Shipping to: United States
Delivery in 1 to 2 Business Days
  • Target
    Binding Specificity
    pThr423
    Reactivity
    Human
    Host
    Rabbit
    Clonality
    Polyclonal
    Conjugate
    Un-conjugated
    Application
    Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Immunogen
    Human PAK 1/2/3 phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
    Isotype
    IgG
  • Application Notes
    This phospho specific polyclonal antibody was tested by ELISA and was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide.   Although not tested, this antibody is likely functional in immunohistochemistry, immunoblotting, and immuno-precipitation.  Lysates from Jurkat cells or PAK transfected cells may be used as a control.This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:25,000 is suggested for this product.  Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide.  Researchers should determine optimal titers for other applications. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) should determine optimal titers for other applications. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:25,000 is suggested for this product.  Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide.  Researchers should determine optimal titers for other applications. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:25,000 is suggested for this product.  Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide.  Researchers should determine optimal titers for other applications. This product has been assayed against 0.1 µg of phosphorylated peptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as a substrate for 30 minutes at room temperature.  A working dilution of 1:5,000 to 1:25,000 is suggested for this product.  Less than 0.2% cross-reactivity was detected against the non-phosphorylated form o f the immunizing peptide.  Researchers should determine optimal titers for other applications.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.94 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
  • Target
    Alternative Name
    PAK 1/2/3 (PAK1/2/3 Antibody Abstract)
    Background
    The p21-activated kinases (PAKs) are a family of multifunctional serine/threonine kinases involved in a variety of cell functions including stress response, apoptosis and regulation of cell motility and tumor metastasis.  Mammalian PAKs are called 1, 2, 3 or a, g, b respectively.  PAKs are part of a large family of kinases where the catalytic domain of the kinase is related to Ste20 kinase of S. cerevisiae.  Pak activity is regulated by specific binding of GTP-bound Rac and cdc42 GTPases and also by sphingosine and related lipids.  PAK1 activation is induced by a variety of growth factors and G-protein-coupled receptors, Fc receptors, and integrins.  This antibody is specific for the phosphorylated form of PAK 1/2/3.  The selected peptide sequence used to generate the polyclonal antibody is common to all human PAKs.
    Synonyms: Alpha PAK antibody, Gamma PAK antibody, Beta PAK antibody
    Gene ID
    5058
    UniProt
    Q13153
You are here: