Phosphotyrosine antibody

Catalog No. ABIN105624

Supplier Product No.: 500-301-262

Product Details anti-Phosphotyrosine Antibody

Target: Phosphotyrosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 500-301-262

Supplier: Rockland

Purpose: Phosphotyrosine Antibody

Cross-Reactivity (Details): Reactivity is specific for phosphotyrosine and minimal cross reactivity is observed against phosphoserine or phosphothreonine.

Characteristics: Synonyms: mouse anti-Phosphotyrosine Antibody, mouse anti-pY antibody, PhosphoTyrosine reactive antibody, monoclonal phosphotyrosine, anti-phosphotyrosine produced in mouse

Purification: This product is clarified ascites produced in balb/c mice using clone 13F9.

Sterility: Sterile filtered

Immunogen:

Immunogen: This monoclonal antibody was produced after repeated immunizations of balb/c mice with phosphotyrosine conjugated KLH.

Immunogen Type: Other

Clone: 13F9

Isotype: IgG1 kappa

Application Details

Application Notes:

Application Note: This monoclonal antibody has been tested in ELISA and Western Blot and reacts specifically with phosphotyrosine and shows minimal reactivity by ELISA and competitive ELISA with phosphoserine or phosphothreonine.  The antibody reacts with free phosphotyrosine, phosphotyrosine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA, flow cytometry, immunohistochemistry and immunoprecipitation.    Phosphorylation of tyrosine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Abstract: Phosphotyrosine Products

Target Type: Amino Acid

Background: Background: Tyrosine (Tyr or Y) or 4-hydroxyphenylalanine is a hydrophilic amino acid. In plants and most microorganisms, tyrosine and α-ketoglutarate are produced via prephenate, when oxidatively decarboxylated with retention of the hydroxyl group to give p-hydroxyphenylpyruvate, which is transaminated using glutamate as the nitrogen source. Mammals synthesize tyrosine from the essential amino acid phenylalanine (phe), which is derived from food. The conversion of phe to tyr is catalyzed by the enzyme phenylalanine hydroxylase causing the addition of a hydroxyl group. In its phosphorylated form, tyrosine is called phosphotyrosine. Tyrosine phosphorylation is one of the key steps in signal transduction and regulation of enzymatic activity. Protein kinases can tag on the hydroxyl group end with a phosphate group to some tyrosine residues causing a phosphorylated form. Tyrosine residues may also be modified by the addition of a sulfate group, a process known as tyrosine sulfation.