HSP90 antibody

Catalog No. ABIN108447

Product Details anti-HSP90 Antibody

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Reactivity: Human, Salmon

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP90 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: fishes, hen, mammals

Characteristics: Expected / apparent Molecular Weight of the Antigene: 84-86 kDa
Antibody has been used on extracts from bovine brain, thymus, muscle, lung,heart, liver, kidney and spleen .

Purification: serum

Immunogen: KLH-conjugated synthetic peptide chosen from a highly conserved region of hsp90 found in both the alpha P07900 and beta P08238 form of the protein. The target peptide is perfectly conserved in animals.

Application Details

Application Notes: 1: 5 000 with standard ECL (WB)

Comment:

in salmonid fish a cross-reactive band at approximately 40 kDa is observed, antibody will also detect a human recombinant HSP90 protein

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-HSP90 antibody (ABIN108447)

Rendell, Fowler, Cockshutt, Currie: "Development-dependent differences in intracellular localization of stress proteins (hsps) in rainbow trout, Oncorhynchus mykiss, following heat shock." in: Comparative biochemistry and physiology. Part D, Genomics & proteomics, Vol. 1, Issue 2, pp. 238-52, (2010) (PubMed).

Target Details for HSP90

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Alternative Name: HSP90 (HSP90 Products)

Background: HSP90 is a ubiquitous chaperone involved in numerous cellular processes. Members of this family of proteins play important roles in allowing a selected group of intracellular signaling molecules reach and maintain functionally active conformations.Alternative names: for hsp90 alpha: HSP 86, renal carcinoma antigen NY-REN-38, for hsp90 beta: hsp84

Molecular Weight: 84-86 kDa

UniProt: P07900, P08238

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals