NEDD9 antibody (AA 82-398)

Catalog No. ABIN108573

The Mouse Monoclonal anti-NEDD9 antibody has been validated for WB, IF, IP, ICC, FACS and IHC (fro). It is suitable to detect NEDD9 in samples from Human, Rat and Mouse. There are 3+ publications available.

Quick Overview for NEDD9 antibody (AA 82-398) (ABIN108573)

Target: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NEDD9 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 2G9

Product Details anti-NEDD9 Antibody

Binding Specificity: AA 82-398

Specificity: Does not recognize p130 Cas. Not tested on Sin1. This antibody mostly detects HEF1 localized at the focal adhesion sites

Purification: Protein G

Immunogen: Fusion protein, corresponding to amino acids 82-398 of Human HEF1.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline try, WB: 1/2000 - 1/6000. Predicted molecular weight: 93 kDa. IP: 1/1000. ICC/IF: Use at an assay dependent dilution. PubMed: 19376971 IHC-Fr: Use at an assay dependent dilution. PubMed: 19464348 Flow Cyt: Use 1 μg for 106 cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Purified antibody containing PBS 0.1 % sodium azide,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

References for anti-NEDD9 antibody (ABIN108573)

Tomkiewicz, Herry, Bui, Métayer, Bourdeloux, Barouki, Coumoul: "The aryl hydrocarbon receptor regulates focal adhesion sites through a non-genomic FAK/Src pathway." in: Oncogene, Vol. 32, Issue 14, pp. 1811-20, (2013) (PubMed).

Zhong, Baquiran, Bonakdar, Lees, Ching, Pugacheva, Fabry, ONeill: "NEDD9 stabilizes focal adhesions, increases binding to the extra-cellular matrix and differentially effects 2D versus 3D cell migration." in: PLoS ONE, Vol. 7, Issue 4, pp. e35058, (2012) (PubMed).

ONeill, Golemis: "Proteolysis of the docking protein HEF1 and implications for focal adhesion dynamics." in: Molecular and cellular biology, Vol. 21, Issue 15, pp. 5094-108, (2001) (PubMed).

Target Details for NEDD9

Target: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Alternative Name: HEF1

Background: Synonyms:
CAS L antibody,Cas like docking antibody,CASL antibody,Crk associated substrate related proteinantibody,dJ49G10.2 antibody,dJ761I2.1 antibody,Enhancer of filamentation 1 antibody,HEF 1 antibody,NEDD 9antibody,NEDD9 antibody,NEDD9 protein antibody,Neural cell expressed developmentally down regulated 9antibody,Neural precursor cell expressed developmentally down regulated 9 antibody,NY REN 12 antigenantibody,p105 antibody,Protein NEDD9 antibody

Gene ID: 4739, 18003, 291044

OMIM: 602265

UniProt: O35177