FASL antibody (Extracellular Domain)

Catalog No. ABIN108724

This Mouse Monoclonal antibody specifically detects FASL in FACS and cELISA. It exhibits reactivity toward Human.

Quick Overview for FASL antibody (Extracellular Domain) (ABIN108724)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FASL antibody is un-conjugated

Application: Flow Cytometry (FACS), Cell-ELISA (cELISA)

Clone: GM-5F4

Product Details anti-FASL Antibody

Binding Specificity: Extracellular Domain

Specificity: GM5F4 recognises FASL transiently expressed on the cell surface of transfected BOSC cells.

Purification: Protein G

Immunogen: genetic immunisation with cDNA encoding human FasL (extracellular domain)

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1.2 µg/10^6 cells
ELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.

Comment:

Synonyms: CD95, Fas ligand

Restrictions: For Research Use only

Handling

Buffer: PBS, pH 7.2

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: short term: 2 °C - 8 °C, long term: -20 °C

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: FASL

Background: The FAS ligand (FASL, CD95), a member of the tumor necrosis factor family, induces apoptosis in FAS-bearing cells. GM5F4 against this transmembrane receptor was generated by genetic immunisation.

UniProt: P48023

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity