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CYP1A antibody

The Mouse Monoclonal anti-CYP1A antibody has been validated for IHC, ELISA and WB. It is suitable to detect CYP1A in samples from Cod.
Catalog No. ABIN108743

Quick Overview for CYP1A antibody (ABIN108743)

Target

CYP1A (Cytochrome P450 1A (CYP1A))

Reactivity

Cod

Host

  • 1
  • 1
Mouse

Clonality

  • 1
  • 1
Monoclonal

Conjugate

  • 2
This CYP1A antibody is un-conjugated

Application

Immunohistochemistry (IHC), ELISA, Western Blotting (WB)

Clone

NP-7
  • Specificity

    The antibody NP-7 cross-reacts with a petroleum/PAH /PCB/dioxin-inducible protein (mr = 55,000-59,000) in liver samples from a wide variety of different fish species from cod (Gadiformes), carp (Cypriniformes), salmon (Salmoniformes), and some flatfish (Pleuronectiformes) (see examples below) . The degree of cross-reactivity may differ between species and with the different methods employed.

    Immunogen

    Immunogen source: Cytochrome P450 1A, purified from liver microsomes of ß-naphthoflavone treated Atlantic cod.

    Isotype

    IgG1 kappa
  • Application Notes

    NP-7 is well suited for a variety of applications including immuno-blotting, ELISA and immunohisto-chemistry. Since assay conditions vary, the optimum dilution should be determined for each particular application. Note: The Antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating. Normal dilution: 1:500 - 1:1000 (Cod). 1:50 - 1:100 (Other species).

    Restrictions

    For Research Use only
  • Format

    Liquid

    Storage

    4 °C
  • Target

    CYP1A (Cytochrome P450 1A (CYP1A))

    Background

    This product consists of Protein-G affinity-purified monoclonal mouse antibodies against CYP1A from Atlantic cod (Gadus morhua). The antibodies are dissolved in 0.1 M Na-phosphate buffer, 0.15 M NaCl, 1% BSA, 0.1% Na-azide, pH 7.4.
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