Follistatin antibody

Catalog No. ABIN1101625

This Rabbit Polyclonal antibody specifically detects Follistatin in WB, ELISA, IHC, IHC (p) and Neut. It exhibits reactivity toward Human.

Quick Overview for Follistatin antibody (ABIN1101625)

Target: Follistatin (FST)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Follistatin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Neutralization (Neut)

Product Details anti-Follistatin Antibody

Brand: IHC-plus™

Specificity: Human Follistatin

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Follistatin.

Type of Immunogen: Cell extract

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), IHC, IHC-P (5 μg/mL), Neut (8 - 12 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: LS-B7434 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 μg/mL is recommended. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human Follistatin (100 ng/mL), a concentration of 8-12 μg/mL of this antibody is required. ELISA: To detect Human Follistatin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Follistatin (LS-C104465) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Follistatin. Western Blot: To detect Human Follistatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Follistatin is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Follistatin

Target: Follistatin (FST)

Alternative Name: FST / Follistatin

Background: Name/Gene ID: FST

Synonyms: FST, Activin-binding protein, Follistatin isoform FST317, Follistatin, Fs

Gene ID: 10468

UniProt: P19883

Pathways: Negative Regulation of Hormone Secretion