P4HB antibody

Catalog No. ABIN1103539

This Mouse Monoclonal antibody specifically detects P4HB in WB, IHC, IP, ICC, FACS, IHC (p), IHC (fro) and Func. It exhibits reactivity toward Human, Mouse, Rat, Hamster and Pig.

Quick Overview for P4HB antibody (ABIN1103539)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivity: Human, Mouse, Rat, Hamster, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This P4HB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Product Details anti-P4HB Antibody

Specificity: Recognizes human, mouse, rat and hamster PDI. Detects a band of ~59kD (rat liver extract) or ~61kD (human liver extract) by Western blot.

Purification: Protein A purified

Immunogen: Purified rat liver PDI (protein disulphide-isomerase).

Type of Immunogen: Purified protein

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo, Func, ICC (1:100), IHC, IHC-Fr, IHC-P (10 μg/mL), IP, WB (1:1000)

Usage: Suitable for use in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (frozen and paraffin), Immunoprecipitation, Western Blot, and Functional application (inhibits PDI activity in vitro). Immunocytochemistry: 1:100. Immunohistochemistry (frozen and paraffin sections): 1:100. Western Blot: 1:1000. Optimal dilution to be determined by the end-user.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 1 mg/mL BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: PDI / P4HB

Background: Name/Gene ID: P4HB

Synonyms: P4HB, ERBA2L, DSI, GIT, p4HB, p4Hbeta, PDIA1, PHDB, PO4DB, PROHB, Protocollagen hydroxylase, Protein disulfide-isomerase, PO4HB, PDI

Gene ID: 5034

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism