PLP1 antibody (C-Term)

Catalog No. ABIN1103681

The Mouse Monoclonal anti-PLP1 antibody has been validated for WB, IHC, IHC (p), IHC (fro) and ICC. It is suitable to detect PLP1 in samples from Human and Mammalian.

Quick Overview for PLP1 antibody (C-Term) (ABIN1103681)

Target: PLP1 (Proteolipid Protein 1 (PLP1))

Reactivity: Human, Mammalian

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-PLP1 Antibody

Binding Specificity: C-Term

Specificity: Reacts with myelin proteolipid protein in many mammalian species. Shows cross-reactivity to DM20.

Purification: Purified

Immunogen: Myelin proteolipid protein, synthetic peptide CGRGTKF corresponding to the C-terminal peptide.

Type of Immunogen: Synthetic peptide

Isotype: IgG2a

Application Details

Application Notes: Approved: ICC, IHC, IHC-Fr, IHC-P (10 μg/mL), WB

Usage: Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot: Can aggregate causing HMW bands to sometimes appear in certain preparations. Immunocytochemistry: 4 % PFA fixed cells for 5-10 minutes at RT. Use 0.1 % triton in block only to allow antibody access to C-terminal epitope. Immunohistochemistry (Frozen): Fresh frozen sections or 4 % PFA fixed, frozen tissues. Epitope is internal, use 0.1-0.2 % triton X-100 in the blocking step for permeabilization. Immunohistochemistry (Paraffin): Works best with lightly fixed tissues, or Bouin's fixed material, although there are reports of traditional formalin fixation also working with antigen recovery. Positive control: Brain.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS buffer,  pH 7.2, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Target Details for PLP1

Target: PLP1 (Proteolipid Protein 1 (PLP1))

Alternative Name: PLP1 / Myelin PLP

Background: Name/Gene ID: PLP1

Synonyms: PLP1, HLD1, PMD, Myelin proteolipid protein, Proteolipid protein 1, SPG2, Lipophilin, MMPL, PLP, PLP/DM20

Gene ID: 5354