TUBA1B antibody (AA 427-441) (Biotin)

Catalog No. ABIN1104869

The Rabbit Polyclonal anti-TUBA1B antibody has been validated for WB, ELISA, IF and IHC. It is suitable to detect TUBA1B in samples from Human, Mouse, Rat, Chicken, Cow, Goat and Sheep.

Quick Overview for TUBA1B antibody (AA 427-441) (Biotin) (ABIN1104869)

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Reactivity: Human, Mouse, Rat, Chicken, Cow, Goat, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUBA1B antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-TUBA1B Antibody (Biotin)

Binding Specificity: AA 427-441

Specificity: A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100 % homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.

Purification: Immunoaffinity purified

Immunogen: Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 427-441 of Human alpha Tubulin.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IF (1:500 - 1:2000), IHC (1:1000 - 1:5000), WB (1:2000 - 1:10000)

Usage: Anti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kD in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA, 0.01 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for TUBA1B

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Alternative Name: TUBA1B

Background: Name/Gene ID: TUBA1B

Synonyms: TUBA1B, Alpha-tubulin ubiquitous, Alpha tubulin, K-ALPHA-1, Tubulin, alpha 1b, Tubulin alpha-ubiquitous chain, Tubulin, alpha, ubiquitous, Tubulin K-alpha-1, Tubulin alpha chain, Tubulin alpha-1B chain

Gene ID: 10376

UniProt: P68363

Pathways: Microtubule Dynamics, M Phase