Actin antibody (C-Term, pan)

Catalog No. ABIN1105235

The Rabbit Polyclonal anti-Actin antibody has been validated for WB and IHC (p). It is suitable to detect Actin in samples from Human and Rat.

Quick Overview for Actin antibody (C-Term, pan) (ABIN1105235)

Target: Actin

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Actin Antibody

Binding Specificity: AA 367-377, C-Term, pan

Sequence: Sequence is identical to the related rat sequence.

Cross-Reactivity (Details): Species reactivity (tested):Human, rat

Purification: Immunogen affinity purified

Immunogen: Synthetic peptide corresponding to a sequence at the C-terminal of human Actin (367-377aa)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for Actin

Target: Actin

Background: Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in the cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. The actins exhibit over 90 % sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three alpha-actin, one beta-actin,two gamma-actin. Because the amino acid sequence of the C-terminal is the same for almost all actins,this antibody has been raised using a synthetic peptide corresponding to the C-terminal 11 residues.