BrdU
IHC, IP, IC
Host: Sheep
Polyclonal
unconjugated
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS containing 50 % glycerol, pH 7.2
Preservative
Azide free
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store (in aliquots) at -20 °C.
Target
BrdU
(Bromodeoxyuridine (BrdU))
Target Type
Chemical
Background
BrdU (5-Bromo-2-Deoxyuridine) is a derivative of uridine that can substitute for thymidine during DNA synthesis. The detection of BrdU incorporation into DNA is a common method to quantify newly synthesized DNA and to identify cells in the S-phase of the cell cycle. BrdU incorporation is frequently used in proliferation assays to study DNA repair, sister chromatid exchange, and the cytokinetics of normal and neoplastic cells.