CD180 antibody

Catalog No. ABIN1105955

The Rat Monoclonal anti-CD180 antibody has been validated for FACS and Func. It is suitable to detect CD180 in samples from Mouse.

Quick Overview for CD180 antibody (ABIN1105955)

Target: CD180 (CD180 Molecule (CD180))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD180 antibody is un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func)

Clone: RP-14

Product Details anti-CD180 Antibody

Cross-Reactivity (Details): Species reactivity (tested):Mouse

Purification: Protein G

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % bovine serum albumin

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.

Expiry Date: 6 months

Target Details for CD180

Target: CD180 (CD180 Molecule (CD180))

Alternative Name: CD180 / RP105

Background: Radioprotective 105 (RP105, CD180) is a type 1 transmembrane protein of 105 kDa with extracellular leucine-rich repeats (LLRs) and a short cytoplasmic tail. RP105, assigned as CD180, is similar to Drosophila Toll in the extracellular LLRs. Radioprotective 105 (RP105, CD180) expression is largely restricted to immune cells including CD19+ B cells and macrophages. Histological studies showed that RP105 is expressed mainly on mature B cells in mantle zones. Antibody-mediated (RP/14) cross-linking of RP105 induces resistance against irradiation-induced apoptosis, B-cell proliferation, and up-regulation of a costimulatory molecule B7.2, revealing RP105 as a potent regulator of B-cell activation. RP105 (CD180) has an important role in B-cell activation by LPS. It is important to note that RP105 associates with MD1, which is in this context important for RP105 with respect to B-cell surface expression and LPS recognition and signalling. MD-1 seems to be requisite for efficient expression of RP105.Synonyms: LY64, Lymphocyte antigen 64, Radioprotective 105 kDa protein

Gene ID: 10090

UniProt: Q62192

Pathways: Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades