CNF1 (C-Term) antibody

Catalog No. ABIN1106726

The Mouse Monoclonal anti- antibody has been validated for WB, Func, DB and EIA. It is suitable to detect in samples from E. coli.

Quick Overview for CNF1 (C-Term) antibody (ABIN1106726)

Target: CNF1

Reactivity: E. coli

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Functional Studies (Func), Dot Blot (DB), Enzyme Immunoassay (EIA)

Clone: NG8

Product Details

Binding Specificity: C-Term

Purification: Protein G purified

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % bovine serum albumin

Storage: 4 °C

Storage Comment: Product should be stored at 2-8 °C.

Target Details

Target: CNF1

Background: CNF1 and CNF2 belong to a family of bacterial toxins that target the small GTP-binding Rho proteins that regulate the actin cytoskeleton. Members of this toxin family typically inactivate Rho, however, CNF1 and the highly related CNF2 activate Rho by deamidation. CNF1 is more frequently associated with E.coli strains that cause extraintestitinal infections in humans, particularly those of the urinary tract (such as cystitis, pyelonephritis and prostatitis). In CNF1-producing uropathogenic E. coli strains, CNF1 is chromosomally encoded and typically resides on a pathogenicity island that also contains hemolysin and P fimbria- related genes. Both CNF1 and the highly related, plasmid-encoded CNF2 are monomeric, cytoplasmic toxins of approximately 115 kDa. CNF1 can be structurally organized into three functional domains the N-terminal, central and the C-terminal domain. The latter exhibits the catalytic activity of the toxin.

UniProt: Q46962